Follicular 17β-estradiol and progesterone concentrations and degree of cumulus cell expansion as predictors of in vivo-matured oocyte developmental competence in superstimulated heifers

• Published in: Theriogenology 2013-10, Vol.80 (6), p.576-583
• Main Authors: Aardema, Hilde, Roelen, Bernard A.J., van Tol, Helena T.A., Oei, Christine H.Y., Gadella, Bart M., Vos, Peter L.A.M.
• Format: Article
• Language: English
• Subjects: 17β-estradiol; Animals; Blastocyst; Cattle; Cell Count; Cell Proliferation; Cumulus Cells - cytology; Cumulus Cells - physiology; Embryonic Development - physiology; Estradiol - analysis; Female; Follicle; follicular fluid; Follicular Fluid - chemistry; heifers; luteinizing hormone; Oocyte developmental competence; oocytes; Oocytes - physiology; Oogenesis - physiology; Ovulation Induction - veterinary; Progesterone; Progesterone - analysis; Prognosis
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2013.05.025

The quality of an oocyte is crucial for successful generation of offspring, but few selection parameters have been identified that reliably predict oocyte developmental competence. The objective of the present study was to determine whether the developmental competence of in vivo-matured oocytes derived from superstimulated heifers could be predicted by 17β-estradiol and progesterone concentrations in follicular fluid, degree of cumulus cell expansion, and follicular diameter. Cumulus oocyte complexes were individually collected from follicles ≥8 mm 22 hours after an induced LH peak and individually fertilized and cultured in vitro. Only oocytes that originated from follicles with 17β-estradiol ≤0.25 μM and progesterone ≥0.26 μM developed into blastocysts. When a combination of these cutoff values was evaluated as a predictor of oocyte competence, the sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 75%, 49%, and 100%, respectively. Hormone concentrations in follicular fluid were also associated with the degree of cumulus cell expansion and only cumulus oocyte complexes with full expansion developed into blastocysts; sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 71%, 45%, and 100%, respectively, when full expansion was used as the predictive criterion for blastocyst production. Follicular diameter was not a good predictor of oocyte competence. In conclusion, concentrations of 17β-estradiol and progesterone in the preovulatory follicle and the degree of cumulus cell expansion are predictors of blastocyst production in superstimulated heifers and can be used as selection markers for oocyte developmental competency.