The effect of minocycline on seizures induced by amygdala kindling in rats

• Published in: Seizure (London, England) England), 2013-10, Vol.22 (8), p.670-674
• Main Authors: Beheshti Nasr, Seyed Mehdi, Moghimi, Ali, Mohammad-Zadeh, Mohammad, Shamsizadeh, Ali, Noorbakhsh, Seyed Mohammad
• Format: Article
• Language: English
• Subjects: Amygdala - drug effects; Amygdala - physiopathology; Animals; Anticonvulsants - pharmacology; Anticonvulsants - therapeutic use; Disease Models, Animal; Electric Stimulation; Kindling; Kindling, Neurologic - drug effects; Minocycline; Minocycline - pharmacology; Minocycline - therapeutic use; Neurology; Rat; Rats; Rats, Wistar; Seizures; Seizures - chemically induced; Seizures - drug therapy; Seizures - physiopathology; Treatment Outcome
• ISSN: 1059-1311; 1532-2688
• DOI: 10.1016/j.seizure.2013.05.005

Abstract Purpose Minocycline is known as a chemical with neuroprotective, anti-inflammatory, and antimicrobial properties. In this study, the effects of minocycline on seizures induced by amygdala kindling in rats were studied. Methods Kindled Wistar rats were injected intraperitoneally with saline and, on the following day, with minocycline (50, 25, and 12.5 mg/kg for the three groups (1–3), respectively). The animals in groups 1–3 had similar protocols. Groups 4 and 5 were given for the rotarod test and received 25 or 50 mg/kg minocycline, respectively, without any kindling stimulation. The animals in groups 6 and 7 (seven each) received 25 mg/kg minocycline or saline, respectively. All the injections were carried out 1 h before kindling stimulation. Seizure parameters, including after discharge duration (ADD), stage 4 latency (S4 L), stage 5 duration (S5 D), and seizure duration (SD), were recorded and compared with those of the saline groups. Results Minocycline (50 mg/kg) significantly reduced ADD, 1/S4 L, S5 D, and SD ( P < 0.001, P < 0.05, P < 0.001, and P < 0.001, respectively) in group 1. While the administration of 25 mg/kg of minocycline decreased the ADD and S5 D ( P < 0.05), in group 2. The injection of 12.5 mg/kg resulted in decreased S5 D ( P < 0.001) in group 3. The daily injection of minocycline (25 mg/kg) significantly decreased ADD, S5 D, and SD ( P < 0.001) in group 6. Conclusion The obtained results revealed that minocycline has anticonvulsant effect on seizures induced by amygdala kindling. Thus, it may be useful for epilepsy treatment.