Systemic Administration of Lipopolysaccharide Increases the Expression of Aquaporin-4 in the Rat Anterior Pituitary Gland

We investigated the effects of lipopolysaccharide (LPS)-induced endotoxemia on the expression of aquaporin-4 (AQP4) in the rat anterior pituitary gland, using the real-time polymerase chain reaction and immunohistochemistry. After intraperitoneal injection of LPS, the level of AQP4 mRNA doubled at 2...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2013, Vol.75(8), pp.1081-1084
Main Authors: KUWAHARA-OTANI, Sachi, MAEDA, Seishi, TANAKA, Koichi, HAYAKAWA, Tetsu, SEKI, Makoto
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
anterior pituitary gland
Aquaporin 4 - metabolism
aquaporin-4
Endotoxemia - chemically induced
Endotoxemia - metabolism
folliculo-stellate cell
Gene Expression Regulation - drug effects
Immunohistochemistry
Lipopolysaccharides - toxicity
Pituitary Gland, Anterior - metabolism
Rats
real-time PCR
Real-Time Polymerase Chain Reaction
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Summary:We investigated the effects of lipopolysaccharide (LPS)-induced endotoxemia on the expression of aquaporin-4 (AQP4) in the rat anterior pituitary gland, using the real-time polymerase chain reaction and immunohistochemistry. After intraperitoneal injection of LPS, the level of AQP4 mRNA doubled at 2, 4 and 8 hr. Immunohistochemical analysis showed an increase with time in AQP4 immunostaining in folliculo-stellate cells following LPS injection; the intensity of immunoreactivity peaked at 8 hr. At the same time, some cyst-like structures, formed by AQP4-positive cells, were observed. These findings indicate that LPS induces the expression of AQP4 in the anterior pituitary gland. The present results should provide an important key to elucidate the pathogenesis of the anterior pituitary gland during endotoxemia.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.13-0083