Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii

A key challenge in the successful treatment of Acanthamoeba infections is its ability to transform into a dormant cyst form that is resistant to physiological conditions and pharmacological therapies, resulting in recurrent infections. The carbohydrate linkage analysis of cyst walls of Acanthamoeba...

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Bibliographic Details
Published in:Parasitology research (1987) 2013-03, Vol.112 (3), p.1221-1227
Main Authors: Aqeel, Yousuf, Siddiqui, Ruqaiyyah, Khan, Naveed Ahmed
Format: Article
Language:English
Subjects:
Acanthamoeba castellanii
Acanthamoeba castellanii - enzymology
Acanthamoeba castellanii - growth & development
Aldose-Ketose Isomerases - antagonists & inhibitors
Aldose-Ketose Isomerases - genetics
Biomedical and Life Sciences
Biomedicine
Gene Silencing
Genetic aspects
Glucosyltransferases - antagonists & inhibitors
Glucosyltransferases - genetics
Immunology
Isomerases
Medical Microbiology
Microbiology
Original Paper
Physiological aspects
Protozoa
RNA sequencing
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Spores, Protozoan - enzymology
Spores, Protozoan - growth & development
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Summary:A key challenge in the successful treatment of Acanthamoeba infections is its ability to transform into a dormant cyst form that is resistant to physiological conditions and pharmacological therapies, resulting in recurrent infections. The carbohydrate linkage analysis of cyst walls of Acanthamoeba castellanii showed variously linked sugar residues, including xylofuranose/xylopyranose, glucopyranose, mannopyranose, and galactopyranose. Here, it is shown that exogenous xylose significantly reduced A . castellanii differentiation in encystation assays ( P  
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-012-3254-6