2-D GEL ELECTROPHORESIS MAP OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS TREATED WITH QUERCUS INFECTORIA GALL EXTRACT

The objective of this study is to determine the optimum concentration of sample protein from Methicillin-Resistant Staphylococcus Aureus (MRSA) after treatment with acetone extract from Quercus infectoria gall. Comparison of the Protein Expression Profile between the treated MRSA and untreated strai...

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Bibliographic Details
Published in:American journal of biochemistry and biotechnology 2013-01, Vol.9 (1), p.19-19
Main Authors: Basri, Dayang Fredalina, Aik, Lee Seng, Khairon, Radhiah, Rahman, Mariati Abdul
Format: Article
Language:English
Subjects:
Quercus
Staphylococcus aureus
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Summary:The objective of this study is to determine the optimum concentration of sample protein from Methicillin-Resistant Staphylococcus Aureus (MRSA) after treatment with acetone extract from Quercus infectoria gall. Comparison of the Protein Expression Profile between the treated MRSA and untreated strain as control was obtained using 2-dimensional gel electrophoresis. The Minimum Inhibitory Concentration (MIC) value of acetone extract of galls from Q. infectoria against two strains of MRSA; ATCC 33591 and PPUKM clinical isolate was determined by broth microdilution method. The MIC value of acetone extracts against both strains of MRSA was 0.3125 mg mL... compared to vancomycin (0.00195 mg mL...). This preliminary study on the PEP of Q. infectoria galls extract-treated MRSA may provide an insight of its antimicrobial mechanism which could lead to the identification of target protein in the future development of a new effective regimen for the treatment of MRSA infections. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:1553-3468
1558-6332