Incorporation of resistance to angular leaf spot and bean common mosaic necrosis virus diseases into adapted common bean (Phaseolus vulgaris L.) genotype in Tanzania

Angular leaf spot (ALS) caused by the fungus Pseudocercospora griseola and Bean common mosaic and necrosis virus (BCMV/BCMNV) are important diseases of common bean in Tanzania that can cause severe yield reduction when uncontrolled. This study was conducted to incorporate resistant genes for ALS and...

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Bibliographic Details
Published in:African journal of biotechnology 2013-07, Vol.12 (27), p.4343-4350
Main Authors: Luseko, Amos Chilagane, George, Muhamba Tryphone, Deogracious, Protas, Elisiana, Kweka, Paul, Mbogo Kusolwa, Susan, Nchimbi Msolla
Format: Article
Language:English
Subjects:
Bean common mosaic necrosis virus
Phaseolus vulgaris
Pseudocercospora
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Summary:Angular leaf spot (ALS) caused by the fungus Pseudocercospora griseola and Bean common mosaic and necrosis virus (BCMV/BCMNV) are important diseases of common bean in Tanzania that can cause severe yield reduction when uncontrolled. This study was conducted to incorporate resistant genes for ALS and BCMV/BCMNV diseases into adapted, market class and farmers and consumers preferred bean genotype using marker assisted selection. The parents Mexico 54 and UBR(25)95 donor of Phg-2 and I/bc-3 genes for ALS and BCMV/BCMNV, respectively were used for the recipient being Kablanketi. In selection, SCAR markers SNO2, ROC11 and SW13 linked to Phg-2, bc-3 and I gene, respectively were used. A parallel backcrossing (modified double cross) procedure was used. The F sub(1), F sub(2) and backcrosses from single crosses were characterized. The Chi square values for ALS were 0.081 (P
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB2012.9651