Quantitative Proteomics Reveals Diverse Roles of miR-148a from Gastric Cancer Progression to Neurological Development

MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify t...

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Published in:Journal of proteome research 2013-09, Vol.12 (9), p.3993-4004
Main Authors: Hu, Chia-Wei, Tseng, Chien-Wei, Chien, Chih-Wei, Huang, Hsuan-Cheng, Ku, Wei-Chi, Lee, Shyh-Jye, Chen, Yu-Ju, Juan, Hsueh-Fen
Format: Article
Language:English
Subjects:
Alzheimer Disease - genetics
Alzheimer Disease - metabolism
Animals
Base Sequence
Brain - embryology
Brain - metabolism
Case-Control Studies
Cell Line, Tumor
Disease Progression
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs - physiology
Parkinson Disease - genetics
Parkinson Disease - metabolism
Proteomics
RNA Interference
Sequence Homology, Nucleic Acid
Spinal Cord - embryology
Spinal Cord - metabolism
Stomach Neoplasms
Transcriptome
Zebrafish
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Summary:MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr400302w