Lypopolysaccharide Downregulates the Expression of Selected Phospholipase C Genes in Cultured Endothelial Cells

The signaling system of phosphoinositides (PI) is involved in a variety of cell and tissue functions, including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation, and cell and tissue polarity. Recently, PI and related molecules, such as the phosphoinositide-specific...

Full description

Saved in:
Bibliographic Details
Published in:Inflammation 2013-08, Vol.36 (4), p.862-868
Main Authors: Lo Vasco, V. R., Leopizzi, M., Chiappetta, C., Puggioni, C., Della Rocca, C., Polonia, P., Businaro, R.
Format: Article
Language:English
Subjects:
Apoptosis
Biomedical and Life Sciences
Biomedicine
Cell Line
Down-Regulation
Gene Expression
Gene Expression Regulation, Enzymologic
Human Umbilical Vein Endothelial Cells - enzymology
Humans
Immunology
Inflammation - chemically induced
Internal Medicine
Lipopolysaccharides - immunology
Pathology
Pharmacology/Toxicology
Phosphatidylinositols - immunology
Phosphoinositide Phospholipase C - genetics
Phosphoinositide Phospholipase C - metabolism
Rheumatology
RNA, Messenger - analysis
Signal Transduction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The signaling system of phosphoinositides (PI) is involved in a variety of cell and tissue functions, including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation, and cell and tissue polarity. Recently, PI and related molecules, such as the phosphoinositide-specific phospholipases C (PI-PLCs), main players in PI signaling were supposed to be involved in inflammation. Besides the control of calcium levels, PI-PLCs contribute to the regulation of phosphatydil-inositol bisphosphate metabolism, crucial in cytoskeletal organization. The expression of PI-PLCs is strictly tissue specific and evidences suggest that it varies under different conditions, such as tumor progression or cell activation. In a previous study, we obtained a complete panel of expression of PI-PLC isoforms in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for endothelial cells. In the present study, we analyzed the mRNA concentration of PI-PLCs in lipopolysaccharide (LPS)-treated HUVEC by using the multiliquid bioanalyzer methodology after 3, 6, 24, 48, and 72 h from LPS administration. Marked differences in the expression of most PI-PLC codifying genes were evident.
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-013-9613-3