Embryonated chicken eggs as an alternative model for mixed Clostridium perfringens and Eimeria tenella infection in chickens

The chorioallantoic membrane (CAM) of chicken embryo eggs is a suitable model for viral and bacterial infections. In the present study, a new approach for testing the pathogenesis and virulence of Clostridium perfringens and Eimeria tenella dual infections as a model using the CAM of embryonated chi...

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Bibliographic Details
Published in:Parasitology research (1987) 2013-06, Vol.112 (6), p.2299-2306
Main Authors: Alnassan, Alaa Aldin, Shehata, Awad Ali, Kotsch, Marianne, Lendner, Matthias, Daugschies, Arwid, Bangoura, Berit
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Chick Embryo
Chickens
Chorioallantoic Membrane - microbiology
Chorioallantoic Membrane - parasitology
Chorioallantoic Membrane - pathology
Clostridium
Clostridium Infections - complications
Clostridium Infections - microbiology
Clostridium Infections - veterinary
Clostridium perfringens
Clostridium perfringens - pathogenicity
Coccidiosis - complications
Coccidiosis - parasitology
Coccidiosis - veterinary
Coinfection - microbiology
Coinfection - parasitology
Coinfection - veterinary
Disease Models, Animal
Egg (Biology)
Eimeria tenella
Eimeria tenella - pathogenicity
Health aspects
Histocytochemistry
Host-parasite relationships
Immunology
Medical Microbiology
Microbiology
Original Paper
Physiological aspects
Poultry Diseases - microbiology
Poultry Diseases - parasitology
Survival Analysis
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Summary:The chorioallantoic membrane (CAM) of chicken embryo eggs is a suitable model for viral and bacterial infections. In the present study, a new approach for testing the pathogenesis and virulence of Clostridium perfringens and Eimeria tenella dual infections as a model using the CAM of embryonated chicken eggs was developed. For this purpose, 24 specific pathogen-free (SPF) embryonated chicken eggs were divided into four groups ( n  = 6) and designated group E, group CP, group CPE, and NC. Sporozoites of E. tenella (20,000 sporozoites) were inoculated into 10-day-old embryonated SPF chicken eggs (groups E and CPE) via allantoic sac route. At 15-day-old, eggs of groups CP and CPE were infected with 10 4  cfu C. perfringens via the same route. Assessment of pathogenicity was assessed using gross and histopathological lesions. Embryo mortality reached 17 % after mono-infection with C. perfringens and/or E. tenella and 50 % in the mixed-infected group. Lesions in the CAMs were most numerous and most severe in co-infected eggs (group CPE), reaching the maximum score of 3 in 50 % of the inoculated eggs ( P  
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-013-3392-5