Spectrofluorimetric and Spectrophotometric Analysis of Two Analgesic Drugs in Pharmaceutical Formulations and Biological Fluids

Simple, reliable, sensitive, and accurate spectrofluorimetric and spectrophotometric methods were proposed for the determination of two selected analgesic drugs, namely tramadol and morphine, in pharmaceuticals and biological fluids. The proposed methods were based on the oxidation of the studied dr...

Full description

Saved in:
Bibliographic Details
Published in:Journal of forensic sciences 2013-09, Vol.58 (5), p.1322-1329
Main Authors: El-Didamony, Akram M., Ali, Ismail I.
Format: Article
Language:English
Subjects:
analgesic drugs
Analgesics
biological samples
Ce(IV)
Cerium
Chemical reactions
Drugs
Dyes
Fluorescence
forensic science
Forensic sciences
Morphine
Oxidation
pharmaceutical formulations
Pharmaceuticals
Scientific imaging
Spectrofluorimetry
Spectrophotometry
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Simple, reliable, sensitive, and accurate spectrofluorimetric and spectrophotometric methods were proposed for the determination of two selected analgesic drugs, namely tramadol and morphine, in pharmaceuticals and biological fluids. The proposed methods were based on the oxidation of the studied drugs by Cerium (Ce)IV in an acidic medium. The spectrofluorimetric method is based on measuring the relative fluorescence intensity of Ce(III) arising from Ce(IV) at 350 nm with an excitation wavelength at 250 nm. The spectrophotometric method involves on addition of a known excess of Ce(IV) to the studied drugs in an acid medium, followed by the determination of residual Ce(IV) by reacting with a fixed amount of methyl orange and measuring the absorbance at 510 nm. Different variables affecting the reaction conditions, such as the concentrations of Ce(IV), type and concentration of the acid medium, reaction time, temperature, and the diluting solvents, were carefully studied and optimized.
ISSN:0022-1198
1556-4029
DOI:10.1111/1556-4029.12170