2-Ketogluconic Acid Secretion by Incorporation of Pseudomonas putida KT 2440 Gluconate Dehydrogenase (gad) Operon in Enterobacter asburiae PSI3 Improves Mineral Phosphate Solubilization

Enterobacter asburiae PSI3 is known to efficiently solubilize rock phosphate by secretion of approximately 50 mM gluconic acid in Tris-buffered medium in the presence of 75 mM glucose and in a mixture of seven aldosugars each at 15 mM concentration, mimicking alkaline vertisol soils. Efficacy of thi...

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Bibliographic Details
Published in:Current microbiology 2013-09, Vol.67 (3), p.388-394
Main Authors: Kumar, Chanchal, Yadav, Kavita, Archana, G, Naresh Kumar, G
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Dehydrogenase
Enterobacter
Enterobacter - genetics
Enterobacter - metabolism
Enterobacter asburiae
Gluconates - metabolism
Gluconic acid
glucose
Life Sciences
Metabolic Engineering - methods
Metabolic Networks and Pathways - genetics
Microbiology
Minerals - metabolism
Operon
Oxidation
Oxidoreductases - genetics
Oxidoreductases - metabolism
Phosphates - metabolism
Phosphorus
Plant growth
Pseudomonas putida
Pseudomonas putida - enzymology
Pseudomonas putida - genetics
Rhizosphere
rhizosphere bacteria
rock phosphate
Rocks
secretion
soil
Sugar
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Summary:Enterobacter asburiae PSI3 is known to efficiently solubilize rock phosphate by secretion of approximately 50 mM gluconic acid in Tris-buffered medium in the presence of 75 mM glucose and in a mixture of seven aldosugars each at 15 mM concentration, mimicking alkaline vertisol soils. Efficacy of this bacterium in the rhizosphere requires P release in the presence of low amount of sugars. To achieve this, E. asburiae PSI3 has been manipulated to express gluconate dehydrogenase (gad) operon of Pseudomonas putida KT 2440 to produce 2-ketogluconic acid. E. asburiae PSI3 harboring gad operon had 438 U of GAD activity, secreted 11.63 mM 2-ketogluconic and 21.65 mM gluconic acids in Tris-rock phosphate-buffered medium containing 45 mM glucose. E. asburiae PSI3 gad transformant solubilized 0.84 mM P from rock phosphate in TRP-buffered liquid medium. In the presence of a mixture of seven sugars each at 12 mM, the transformant brought about a drop in pH to 4.1 and released 0.53 mM P.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-013-0372-z