recombinant plasmid carrying the mitochondrial plasmid sequence of Neurospora intermedia LaBelle yields new plasmid derivatives in Neurospora crassa transformants

The authors have studied the efficacy of transformation of Neurospora crassa with a chimaeric plasmid. They constructed a recombinant plasmid, pMK2, consisting of the mitochondrial plasmid of N. intermedia LaBelle, a part of the qa gene cluster of Neurospora and Escherichia coli plasmid pBR322. Comp...

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Bibliographic Details
Published in:Current genetics 1985-01, Vol.9 (6), p.471-477
Main Authors: Kuper, M.T.R, Vries, H. de
Format: Article
Language:English
Subjects:
Neurospora
Neurospora crassa
Neurospora intermedia
plasmids
transformation
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Summary:The authors have studied the efficacy of transformation of Neurospora crassa with a chimaeric plasmid. They constructed a recombinant plasmid, pMK2, consisting of the mitochondrial plasmid of N. intermedia LaBelle, a part of the qa gene cluster of Neurospora and Escherichia coli plasmid pBR322. Compared to plasmid pVK88, not containing the LaBelle sequence, the pMK2 plasmid gives a five-fold increase in transformation of the qa 2 super(+) gene. Analysis of the DNA from Neurospora) transformants revealed that the pMK2 plasmid is not stable. The qa insert as well as the LaBelle part of pMK2 are rapidly lost from the plasmid. In most cases the qa insert integrates into the nuclear DNA of the host. Plasmids recovered from Neurospora transformants are rearranged and show insertions or deletions. Some of these plasmids are described here.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00434052