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TUNEL analysis of DNA fragmentation in mouse unfertilized oocytes: The effect of microorganisms within human follicular fluid collected during IVF cycles
Abstract Recently we reported the presence of bacteria within follicular fluid. Previous studies have reported that DNA fragmentation in human spermatozoa after in vivo or in vitro incubation with bacteria results in early embryo demise and a reduced rate of ongoing pregnancy, but the effect of bact...
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| Published in: | Journal of reproductive immunology 2013-09, Vol.99 (1), p.69-79 |
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| Main Authors: | , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c408t-9234fcb501b83b591bffaf3aaebb70d9b4478d7a5c3640ce61392a6795987b93 |
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| container_start_page | 69 |
| container_title | Journal of reproductive immunology |
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| creator | Pelzer, Elise S Harris, Jessica E Allan, John A Waterhouse, Mary A Ross, Tara Beagley, Kenneth W Knox, Christine L |
| description | Abstract Recently we reported the presence of bacteria within follicular fluid. Previous studies have reported that DNA fragmentation in human spermatozoa after in vivo or in vitro incubation with bacteria results in early embryo demise and a reduced rate of ongoing pregnancy, but the effect of bacteria on oocytes is unknown. This study examined the DNA within mouse oocytes after 12 hours’ incubation within human follicular fluids ( n = 5), which were collected from women undergoing in vitro fertilization (IVF) treatment. Each follicular fluid sample was cultured to detect the presence of bacteria. Terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) was used to label DNA fragmentation in ovulated, non-fertilized mouse oocytes following in vitro incubation in human follicular fluid. The bacteria Streptococcus anginosus and Peptoniphilus spp., Lactobacillus gasseri (low-dose), L. gasseri (high-dose), Enterococcus faecalis , or Propionibacterium acnes were detected within the follicular fluids. The most severe DNA fragmentation was observed in oocytes incubated in the follicular fluids containing P. acnes or L. gasseri (high-dose). No DNA fragmentation was observed in the mouse oocytes incubated in the follicular fluid containing low-dose L. gasseri or E. faecalis . Low human oocyte fertilization rates ( |
| doi_str_mv | 10.1016/j.jri.2013.07.004 |
| format | article |
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Previous studies have reported that DNA fragmentation in human spermatozoa after in vivo or in vitro incubation with bacteria results in early embryo demise and a reduced rate of ongoing pregnancy, but the effect of bacteria on oocytes is unknown. This study examined the DNA within mouse oocytes after 12 hours’ incubation within human follicular fluids ( n = 5), which were collected from women undergoing in vitro fertilization (IVF) treatment. Each follicular fluid sample was cultured to detect the presence of bacteria. Terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) was used to label DNA fragmentation in ovulated, non-fertilized mouse oocytes following in vitro incubation in human follicular fluid. The bacteria Streptococcus anginosus and Peptoniphilus spp., Lactobacillus gasseri (low-dose), L. gasseri (high-dose), Enterococcus faecalis , or Propionibacterium acnes were detected within the follicular fluids. The most severe DNA fragmentation was observed in oocytes incubated in the follicular fluids containing P. acnes or L. gasseri (high-dose). No DNA fragmentation was observed in the mouse oocytes incubated in the follicular fluid containing low-dose L. gasseri or E. faecalis . Low human oocyte fertilization rates (<29%) were associated with extensive fragmentation in mouse oocytes (80–100%). Bacteria colonizing human follicular fluid in vivo may cause DNA fragmentation in mouse oocytes following 12 h of in vitro incubation. Follicular fluid bacteria may result in poor quality oocytes and/or embryos, leading to poor IVF outcomes.</description><identifier>ISSN: 0165-0378</identifier><identifier>EISSN: 1872-7603</identifier><identifier>DOI: 10.1016/j.jri.2013.07.004</identifier><identifier>PMID: 23972717</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adult ; Animals ; Bacteria ; Bacterial Infections - complications ; Bacterial Infections - genetics ; Bacterial Infections - microbiology ; Cells, Cultured ; Disease Models, Animal ; DNA Fragmentation ; Female ; Fertilization ; Fertilization in Vitro ; Follicular fluid ; Follicular Fluid - metabolism ; Follicular Fluid - microbiology ; Humans ; In Situ Nick-End Labeling ; In vitro fertilization ; Infertility, Female - complications ; Infertility, Female - microbiology ; Infertility, Female - therapy ; Mice ; Mouse oocytes ; Obstetrics and Gynecology ; Oocytes - metabolism ; Oocytes - microbiology ; Pregnancy ; Treatment Outcome</subject><ispartof>Journal of reproductive immunology, 2013-09, Vol.99 (1), p.69-79</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2013 Elsevier Ireland Ltd</rights><rights>Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-9234fcb501b83b591bffaf3aaebb70d9b4478d7a5c3640ce61392a6795987b93</citedby><cites>FETCH-LOGICAL-c408t-9234fcb501b83b591bffaf3aaebb70d9b4478d7a5c3640ce61392a6795987b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23972717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pelzer, Elise S</creatorcontrib><creatorcontrib>Harris, Jessica E</creatorcontrib><creatorcontrib>Allan, John A</creatorcontrib><creatorcontrib>Waterhouse, Mary A</creatorcontrib><creatorcontrib>Ross, Tara</creatorcontrib><creatorcontrib>Beagley, Kenneth W</creatorcontrib><creatorcontrib>Knox, Christine L</creatorcontrib><title>TUNEL analysis of DNA fragmentation in mouse unfertilized oocytes: The effect of microorganisms within human follicular fluid collected during IVF cycles</title><title>Journal of reproductive immunology</title><addtitle>J Reprod Immunol</addtitle><description>Abstract Recently we reported the presence of bacteria within follicular fluid. Previous studies have reported that DNA fragmentation in human spermatozoa after in vivo or in vitro incubation with bacteria results in early embryo demise and a reduced rate of ongoing pregnancy, but the effect of bacteria on oocytes is unknown. This study examined the DNA within mouse oocytes after 12 hours’ incubation within human follicular fluids ( n = 5), which were collected from women undergoing in vitro fertilization (IVF) treatment. Each follicular fluid sample was cultured to detect the presence of bacteria. Terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) was used to label DNA fragmentation in ovulated, non-fertilized mouse oocytes following in vitro incubation in human follicular fluid. The bacteria Streptococcus anginosus and Peptoniphilus spp., Lactobacillus gasseri (low-dose), L. gasseri (high-dose), Enterococcus faecalis , or Propionibacterium acnes were detected within the follicular fluids. The most severe DNA fragmentation was observed in oocytes incubated in the follicular fluids containing P. acnes or L. gasseri (high-dose). No DNA fragmentation was observed in the mouse oocytes incubated in the follicular fluid containing low-dose L. gasseri or E. faecalis . Low human oocyte fertilization rates (<29%) were associated with extensive fragmentation in mouse oocytes (80–100%). Bacteria colonizing human follicular fluid in vivo may cause DNA fragmentation in mouse oocytes following 12 h of in vitro incubation. Follicular fluid bacteria may result in poor quality oocytes and/or embryos, leading to poor IVF outcomes.</description><subject>Adult</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacterial Infections - complications</subject><subject>Bacterial Infections - genetics</subject><subject>Bacterial Infections - microbiology</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>DNA Fragmentation</subject><subject>Female</subject><subject>Fertilization</subject><subject>Fertilization in Vitro</subject><subject>Follicular fluid</subject><subject>Follicular Fluid - metabolism</subject><subject>Follicular Fluid - microbiology</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>In vitro fertilization</subject><subject>Infertility, Female - complications</subject><subject>Infertility, Female - microbiology</subject><subject>Infertility, Female - therapy</subject><subject>Mice</subject><subject>Mouse oocytes</subject><subject>Obstetrics and Gynecology</subject><subject>Oocytes - metabolism</subject><subject>Oocytes - microbiology</subject><subject>Pregnancy</subject><subject>Treatment Outcome</subject><issn>0165-0378</issn><issn>1872-7603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9ksFu1DAURSMEokPhA9ggL9kkPMdJnICEVJUWKo3KgoGt5TjPMx4cu9gJKPwJf4ujKSxYsLJk3XOl6-Mse06hoECbV8fiGExRAmUF8AKgepBtaMvLnDfAHmablKlzYLw9y57EeASgHDr6ODsrWcdLTvkm-7X7fHu1JdJJu0QTidfk3e0F0UHuR3STnIx3xDgy-jkimZ3GMBlrfuJAvFfLhPE12R2QoNaophUfjQreh710Jo6R_DDTIfGHeZSOaG-tUbOVgWg7m4GodJG41DbMwbg9uflyTdSiLMan2SMtbcRn9-d5tru-2l1-yLcf399cXmxzVUE75V3JKq36Gmjfsr7uaK-11ExK7HsOQ9dXFW8HLmvFmgoUNpR1pWx4V3ct7zt2nr081d4F_23GOInRRIXWSodps6AVow3tWmApSk_RNDDGgFrcBTPKsAgKYhUijiIJEasQAVwkIYl5cV8_9yMOf4k_BlLgzSmAaeN3g0FEZdApHExILyMGb_5b__YfWlnjjJL2Ky4Yj34OyWxaIWIpQHxaf8T6ISgDgJZR9hvKMrL0</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Pelzer, Elise S</creator><creator>Harris, Jessica E</creator><creator>Allan, John A</creator><creator>Waterhouse, Mary A</creator><creator>Ross, Tara</creator><creator>Beagley, Kenneth W</creator><creator>Knox, Christine L</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130901</creationdate><title>TUNEL analysis of DNA fragmentation in mouse unfertilized oocytes: The effect of microorganisms within human follicular fluid collected during IVF cycles</title><author>Pelzer, Elise S ; Harris, Jessica E ; Allan, John A ; Waterhouse, Mary A ; Ross, Tara ; Beagley, Kenneth W ; Knox, Christine L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-9234fcb501b83b591bffaf3aaebb70d9b4478d7a5c3640ce61392a6795987b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacterial Infections - complications</topic><topic>Bacterial Infections - genetics</topic><topic>Bacterial Infections - microbiology</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>DNA Fragmentation</topic><topic>Female</topic><topic>Fertilization</topic><topic>Fertilization in Vitro</topic><topic>Follicular fluid</topic><topic>Follicular Fluid - metabolism</topic><topic>Follicular Fluid - microbiology</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>In vitro fertilization</topic><topic>Infertility, Female - complications</topic><topic>Infertility, Female - microbiology</topic><topic>Infertility, Female - therapy</topic><topic>Mice</topic><topic>Mouse oocytes</topic><topic>Obstetrics and Gynecology</topic><topic>Oocytes - metabolism</topic><topic>Oocytes - microbiology</topic><topic>Pregnancy</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pelzer, Elise S</creatorcontrib><creatorcontrib>Harris, Jessica E</creatorcontrib><creatorcontrib>Allan, John A</creatorcontrib><creatorcontrib>Waterhouse, Mary A</creatorcontrib><creatorcontrib>Ross, Tara</creatorcontrib><creatorcontrib>Beagley, Kenneth W</creatorcontrib><creatorcontrib>Knox, Christine L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of reproductive immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pelzer, Elise S</au><au>Harris, Jessica E</au><au>Allan, John A</au><au>Waterhouse, Mary A</au><au>Ross, Tara</au><au>Beagley, Kenneth W</au><au>Knox, Christine L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TUNEL analysis of DNA fragmentation in mouse unfertilized oocytes: The effect of microorganisms within human follicular fluid collected during IVF cycles</atitle><jtitle>Journal of reproductive immunology</jtitle><addtitle>J Reprod Immunol</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>99</volume><issue>1</issue><spage>69</spage><epage>79</epage><pages>69-79</pages><issn>0165-0378</issn><eissn>1872-7603</eissn><abstract>Abstract Recently we reported the presence of bacteria within follicular fluid. Previous studies have reported that DNA fragmentation in human spermatozoa after in vivo or in vitro incubation with bacteria results in early embryo demise and a reduced rate of ongoing pregnancy, but the effect of bacteria on oocytes is unknown. This study examined the DNA within mouse oocytes after 12 hours’ incubation within human follicular fluids ( n = 5), which were collected from women undergoing in vitro fertilization (IVF) treatment. Each follicular fluid sample was cultured to detect the presence of bacteria. Terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) was used to label DNA fragmentation in ovulated, non-fertilized mouse oocytes following in vitro incubation in human follicular fluid. The bacteria Streptococcus anginosus and Peptoniphilus spp., Lactobacillus gasseri (low-dose), L. gasseri (high-dose), Enterococcus faecalis , or Propionibacterium acnes were detected within the follicular fluids. The most severe DNA fragmentation was observed in oocytes incubated in the follicular fluids containing P. acnes or L. gasseri (high-dose). No DNA fragmentation was observed in the mouse oocytes incubated in the follicular fluid containing low-dose L. gasseri or E. faecalis . Low human oocyte fertilization rates (<29%) were associated with extensive fragmentation in mouse oocytes (80–100%). Bacteria colonizing human follicular fluid in vivo may cause DNA fragmentation in mouse oocytes following 12 h of in vitro incubation. Follicular fluid bacteria may result in poor quality oocytes and/or embryos, leading to poor IVF outcomes.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>23972717</pmid><doi>10.1016/j.jri.2013.07.004</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0165-0378 |
| ispartof | Journal of reproductive immunology, 2013-09, Vol.99 (1), p.69-79 |
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| language | eng |
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| source | Elsevier |
| subjects | Adult Animals Bacteria Bacterial Infections - complications Bacterial Infections - genetics Bacterial Infections - microbiology Cells, Cultured Disease Models, Animal DNA Fragmentation Female Fertilization Fertilization in Vitro Follicular fluid Follicular Fluid - metabolism Follicular Fluid - microbiology Humans In Situ Nick-End Labeling In vitro fertilization Infertility, Female - complications Infertility, Female - microbiology Infertility, Female - therapy Mice Mouse oocytes Obstetrics and Gynecology Oocytes - metabolism Oocytes - microbiology Pregnancy Treatment Outcome |
| title | TUNEL analysis of DNA fragmentation in mouse unfertilized oocytes: The effect of microorganisms within human follicular fluid collected during IVF cycles |
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