Presence of auto-antibody against two placental proteins, annexin A1 and vitamin D binding protein, in sera of women with pre-eclampsia

• Published in: Journal of reproductive immunology 2013-09, Vol.99 (1), p.10-16
• Main Authors: Behrouz, Gharesi-Fard, Farzaneh, Ghaderi-shabankareh, Leila, Jafarzadeh, Jaleh, Zolghadri, Eskandar, Kamali-Sarvestani
• Format: Article
• Language: English
• Subjects: Adult; Annexin A1; Annexin A1 - immunology; Autoantibodies - blood; Autoantigens - metabolism; Autoimmunity; Female; Humans; Mass Spectrometry; Obstetrics and Gynecology; Placenta; Pre-eclampsia; Pre-Eclampsia - immunology; Pre-Eclampsia - therapy; Pregnancy; Pregnancy Proteins - immunology; Pregnancy Proteins - isolation & purification; Proteomics; Vitamin D binding protein; Vitamin D-Binding Protein - immunology; Young Adult
• ISSN: 0165-0378; 1872-7603
• DOI: 10.1016/j.jri.2013.04.007

Abstract Pre-eclampsia (PE) is one of the most complex and life-threatening pregnancy disorders. PE is characterized by maternal hypertension and proteinuria. There is much evidence to support an immunological etiology for PE and auto-immunity is considered a predisposing factor for PE. The aim of the present study was the investigation of placental proteins as targets for auto-antibodies in PE patients. 2D-PAGE technique was used for separation of the total human placental proteins. After separation, protein spots were transferred to the PVDF membranes and blotted with sera from 20 PE patients and compared with membranes blotted with 20 sera from normal women. MALDI TOF/TOF mass spectrometry technique was used for identification of differentially blotted spots. Moreover, the results of mass analysis were confirmed using western blot with commercial mAbs and RT-PCR technique. The results indicated that two placental proteins, annexin A1 and vitamin D binding protein (DBP), might be targeted by PE sera. The expression of annexin A1 and DBP was also confirmed at RNA level using the RT-PCR technique. Furthermore, the mass results were confirmed by western blotting with commercial mAbs against two targeted proteins. The data of the present study suggest two new placental proteins, annexin A1 and DBP, as placental immune targets. Considering the relation among vitamin D deficiency, increased risk of PE, and the role of annexin A1 in the resolution of inflammation, production of antibody against annexin A1 and DBP may be considered a new auto-immune hypothesis in pre-eclampsia that calls for further investigation in future work.