Molecular characterization of the antimicrobial resistance of Riemerella anatipestifer isolated from ducks

• Published in: Veterinary microbiology 2012-08, Vol.158 (3-4), p.376-383
• Main Authors: Sun, Na, Liu, Jian-Hua, Yang, Fan, Lin, Da-Chuan, Li, Guang-Hui, Chen, Zhang-Liu, Zeng, Zhen-Ling
• Format: Article
• Language: English
• Subjects: amikacin; amino acids; ampicillin; Animals; Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; antibiotic resistance; apramycin; Bacteriology; Biological and medical sciences; China; ciprofloxacin; drug effects; Drug Resistance, Bacterial; Drug Resistance, Bacterial - genetics; Drug Resistance, Multiple, Bacterial; Drug Resistance, Multiple, Bacterial - genetics; ducks; Ducks - microbiology; farms; florfenicol; Fundamental and applied biological sciences. Psychology; genes; Genes, Bacterial; Genes, Bacterial - genetics; genetics; gentamicin; Integron; Integrons; Integrons - genetics; isolation & purification; Microbial Sensitivity Tests; Microbiology; Miscellaneous; nalidixic acid; neomycin; pharmacology; Plasmids; Plasmids - genetics; point mutation; polymerase chain reaction; QRDR mutation; Quinolone; Resistance genes; resistance mechanisms; Riemerella; Riemerella - drug effects; Riemerella - genetics; Riemerella - isolation & purification; Riemerella anatipestifer; streptomycin; sulfamethazine
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2012.03.005

The antimicrobial susceptibility of 103 Riemerella anatipestifer isolates obtained from ducks during 2008 and 2010 in Southern China, to 23 antimicrobial agents was investigated using the agar dilution method. The MIC50 and MIC90 values of streptomycin, kanamycin, gentamicin, apramycin, amikacin, neomycin, nalidixic acid and sulfadimidine were high (32–≥128μg/ml) among the 103 R. anatipestifer isolates. However, relatively low MIC90 values (8μg/ml) of ampicillin and florfenicol were observed among these isolates. The presence of resistance genes and integrons was determined using PCR. The genes blaTEM-1, aph(3′)-VII, aadA1, aadA2, aac(3′)-IV, aac(3′)-IIc, aac(6′)-Ib, cat2, cmlA, floR, tet(A), tet(B), tet(C), sul1, and sul2 were detected in 1, 2, 24, 35, 11, 4, 67, 16, 26, 10, 6, 1, 9, 36 and 2 isolates, respectively. Twenty isolates contained one or two class 1 integrons carrying aadA2 or aac(6′)-II-catB3-aadA1 gene cassette(s). Mutation analysis of the quinolone resistance-determining regions (QRDRs) of 43 R. anatipestifer isolates with nalidixic acid MICs≥32μg/ml, showed that the most prevalent mutations in gyrA were those resulting in the amino acid exchanges Ser83-Ile (n=37), followed by Asp87-His (n=7) and Ser83-Arg (n=5). Point mutations in parC (Arg120-Glu) were observed in 5 isolates with a ciprofloxacin MIC of >16μg/ml. No plasmid-mediated quinolone resistance gene was detected. PFGE analysis showed that the clonal spread of multi-drug resistant R. anatipestifer isolates occurred in the same farm or between different farms. Our results reported, for the first time, the mechanism of quinolone resistance in R. anatipestifer.