Serum specific IgA antibody to Chlamydia trachomatis in patients with chlamydial infections detected by ELISA and an immunofluorescence test

Sera obtained from 34 men with Chlamydia trachomatis positive non-gonococcal urethritis, 34 men with C. trachomatis negative non-gonococcal urethritis, 42 women with acute salpingitis, 38 healthy women, and 34 healthy men were studied for the presence of specific serum C. trachomatis IgA and IgG ant...

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Bibliographic Details
Published in:Journal of clinical pathology 1984-01 (37), p.686-691
Main Authors: Cevenini, R, Sarov, I, Rumpianesi, F, Donati, M, Melega, C, Varotti, C, La Placa, M
Format: Article
Language:English
Subjects:
Chlamydia trachomatis
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Summary:Sera obtained from 34 men with Chlamydia trachomatis positive non-gonococcal urethritis, 34 men with C. trachomatis negative non-gonococcal urethritis, 42 women with acute salpingitis, 38 healthy women, and 34 healthy men were studied for the presence of specific serum C. trachomatis IgA and IgG antibodies. Serological results were correlated with C. trachomatis isolation in cell culture. An enzyme linked immunosorbent assay (ELISA) for C. trachomatis specific serum IgA was employed using highly purified elementary bodies of C. trachomatis serotype L2 grown in LLC-MK2 cells. Results obtained for C. trachomatis IgA antibody by the ELISA test were compared with the results obtained for the same sera by a single antigen immunofluorescence technique. A good correlation was found between two methods. Serum IgG antibody was also determined in the same sera by the immunofluorescence technique.
ISSN:0021-9746