Tonometry as a predictor of inadequate splanchnic perfusion in an intra-abdominal hypertension animal model

• Published in: The Journal of surgical research 2013-10, Vol.184 (2), p.1028-1034
• Main Authors: Correa-Martín, Laura, DVM, PhD, Castellanos, Gregorio, MD, PhD, García-Lindo, Mónica, DVM, Díaz-Güemes, Idoia, DVM, PhD, Sánchez-Margallo, Francisco M., DVM, PhD
• Format: Article
• Language: English
• Subjects: Abdominal compartment syndrome; Animals; Blood Pressure - physiology; Disease Models, Animal; Female; Hemodynamics - physiology; Histologic examination; Intestine, Small - blood supply; Intestine, Small - pathology; Intra-Abdominal Hypertension - diagnosis; Intra-Abdominal Hypertension - physiopathology; Intraabdominal hypertension; Manometry - methods; Porcine model; Predictive Value of Tests; Splanchnic Circulation - physiology; Surgery; Swine; Tonometry
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2013.04.041

Abstract Background The gastrointestinal system is the most sensitive to the presence of intra-abdominal hypertension. We aimed to assess the early prognostic value of gastric air tonometry as a predictor of inadequate splanchnic perfusion and determine its relation with abdominal perfusion pressure (APP). Methods Twenty-five Large White swine were used for this study. A control group and two study groups were included, in which intra-abdominal pressure (IAP) was elevated with Co2 to 20 and 30 mmHg during 5 h. We measured the intramucosal gastric pH (pHim) and determined gastric luminal PCO2 (PgCO2 ) and PgCO2 gap (gastric luminal CO2 –arterial CO2 ) to evaluate gastric acidity. APP was indirectly obtained through IAP and mean arterial pressure. Additionally, histopathologic samples of small intestine were obtained and analyzed. Results pHim showed a decrease in IAP groups, with statistical significance in the 30 mmHg group, 90 min after stabilization period ( P < 0.01). Serum lactate showed delayed alteration when compared with pHim, with significant increase, 180 min after stabilization ( P < 0.05). The values of PgCO2 and PCO2 gap were increased in IAP groups, being statistically significant in the 30 mmHg group, 120 and 150 min, respectively, after stabilization. In increased IAP groups, there was a time progressive decrease of APP, with statistically significant differences observed between groups at 20 min ( P < 0.001). The histopathology study revealed parenchymal injury of the intestine at 30 mmHg. Conclusions Tonometry is sensitive to the increase in IAP and relates to the reduction of APP generated by splanchnic hypoperfusion.