Acid-Base Parameters and Steroid Concentrations in Pre-Ovulatory Follicles and Plasma of Lactating Dairy Cows with Spontaneous and Synchronized Oestrus or Follicular Cyst

• Published in: Reproduction in domestic animals 2013-10, Vol.48 (5), p.833-839
• Main Authors: Hussein, HA, Boryczko, Z, Bostedt, H
• Format: Article
• Language: English
• Subjects: Acid-Base Equilibrium; Acids; Androstenedione - blood; Androstenedione - metabolism; Animal reproduction; Animals; Breastfeeding & lactation; Cattle; Cattle Diseases - blood; Cysts; Estradiol - blood; Estradiol - metabolism; Estrus - physiology; Estrus Synchronization; Female; Follicular Cyst - metabolism; Lactation; Ovarian Follicle; Parameter estimation; Plasma; Progesterone - blood; Progesterone - metabolism; Steroids
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.12171

Contents The study aimed to compare the acid–base balance and steroid concentrations between follicular fluids (FF) of pre‐ovulatory follicles derived from a spontaneous oestrus (SO), synchronized or induced oestrus (IO) and follicular cysts (CYS) and between FF and blood in dairy cows. Forty‐two dairy cows were included in this study. The animals were allocated to three groups: SO (n = 23); IO (n = 11) using GnRH at day 0 and day 9 and PGF2α at day 7; and animals with CYS (n = 10). The follicular fluids (FF) were aspirated from the cyst/pre‐ovulatory follicles (∅ ≥ 15 mm) after SO and after second GnRH dose in IO by transvaginal ultrasound‐guided ovum pick‐up technique. Blood samples (BL) were collected in heparinized vacutainer tubes. The oxygen tension (pO2) in FF of IO was higher (p