Multiplex ligation-dependent probe amplification (MLPA)--new possibilities of prenatal diagnosis

Multiplex Ligation-dependent Probe Amplification (MLPA) is a relatively new method of molecular diagnosis. It enables a relative quantitative assessment of up to 50 different PCR amplicons in one reaction by the use of a very small amount of examined DNA. Nowadays MLPA is becoming a very helpful too...

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Bibliographic Details
Published in:Ginekologia polska 2013-06, Vol.84 (6), p.461-464
Main Authors: Massalska, Diana, Bijok, Julia, Zimowski, Janusz G, Jóźwiak, Anna, Jakiel, Grzegorz, Roszkowski, Tomasz
Format: Article
Language:English
Subjects:
Chromosome Disorders - diagnosis
Female
Humans
Multiplex Polymerase Chain Reaction - methods
Pregnancy
Prenatal Care - methods
Prenatal Diagnosis - methods
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Summary:Multiplex Ligation-dependent Probe Amplification (MLPA) is a relatively new method of molecular diagnosis. It enables a relative quantitative assessment of up to 50 different PCR amplicons in one reaction by the use of a very small amount of examined DNA. Nowadays MLPA is becoming a very helpful tool in prenatal diagnosis and is widely used for the detection of aneuploidies, familial single gene disorders, common microdeletion syndromes, sub-telomeric alterations and identification of marker chromosomes in fetuses. This review demonstrates possible applications of MLPA in prenatal diagnosis.
ISSN:0017-0011
0017-0011
2543-6767
DOI:10.17772/gp/1605