Isolation and Characterization of Pseudomonas sp. STM 997 from Soil Sample having Potentiality to Degrade 3,6-Dimethyl-1-keto-1,2,3,4-tetrahydrocarbazole: A Novel Approach

• Published in: Applied biochemistry and biotechnology 2012-12, Vol.168 (7), p.1765-1777
• Main Authors: Chakraborty, Biswanath, Chakraborty, Suchandra, Basu, Anjan Kumar, Aditya, Bhrigu, Sinha, T. P., Dhar, Tanima Modak, Saha, Chandan
• Format: Article
• Language: English
• Subjects: Alkylation; Base Sequence; Biochemical analysis; Biochemistry; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; Bioremediation; Biotechnology; Biotransformation; Carbazoles - chemistry; Carbazoles - isolation & purification; Carbazoles - metabolism; Carbon - metabolism; Chemistry; Chemistry and Materials Science; Energy Metabolism; Environmental Pollutants - chemistry; Environmental Pollutants - isolation & purification; Environmental Pollutants - metabolism; Fundamental and applied biological sciences. Psychology; Liquid chromatography; Molecular Sequence Data; Pseudomonas; Pseudomonas - genetics; Pseudomonas - isolation & purification; Pseudomonas - metabolism; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Soil contaminants; Soil contamination; Soil Microbiology; Thin layer chromatography
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-012-9895-1

A pure colony of a bacterium from contaminated soil was isolated by exploiting 3,6-dimethyl-1-keto-1,2,3,4–tetrahydrocarbazole, a novel carbazole derivative, having indole moiety as well as 3-methyl functionality both in aromatic and hydro-aromatic moiety, as a sole source of carbon and energy. Taxonomical studies, biochemical analysis, and 16S rDNA sequence analysis indicated that the isolated strain has close similarity with Pseudomonas sp. Thin-layer chromatography followed by HPLC and mass spectroscopic study indicates that the isolated Pseudomonas sp. STM 997 degrades 3,6-dimethyl-1-keto-1,2,3,4–tetrahydrocarbazole, and this strain may be useful in the bioremediation of environments contaminated by the compounds containing carbazole moiety with methyl substituents at various reactive sites. This study also provides an evidence in favor of the suggested biodegradation of 3-methylcarbazole to carbazole in plants.