Genetic and phenotypic characterization of a Japanese wild-derived DOB/Oda rat strain

Wild-derived rat strains can provide novel genome resources that are not available in standard laboratory strains. Genetic backgrounds of wild-derived strains can facilitate effective genetic linkage analyses and often modulate the expression of mutant phenotypes. Here we describe the development an...

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Bibliographic Details
Published in:Mammalian genome 2013-08, Vol.24 (7-8), p.303-308
Main Authors: Kuramoto, Takashi, Inoue, Satoko, Neoda, Yuki, Yamasaki, Ken-ichi, Hashimoto, Ryoko, Mashimo, Tomoji, Oda, Sen-ichi, Serikawa, Tadao
Format: Article
Language:English
Subjects:
alleles
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
body weight
Body Weight - genetics
Breeding
Cell Biology
Female
Genome - genetics
Genotype
Human Genetics
Japan
Life Sciences
locomotion
Male
mutants
Phenotype
phylogeny
Polymorphism, Genetic
Rats
Rats, Inbred Strains - genetics
Rattus norvegicus
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Summary:Wild-derived rat strains can provide novel genome resources that are not available in standard laboratory strains. Genetic backgrounds of wild-derived strains can facilitate effective genetic linkage analyses and often modulate the expression of mutant phenotypes. Here we describe the development and characterization of a new inbred rat strain, DOB/Oda, from wild rats (Rattus norvegicus) captured in Shitara, Aichi, Japan. Phenotype analysis of 109 parameters revealed that the DOB/Oda rats had small body weight, preference for darkness, and high locomotor activity compared with the rat strains in the National BioResource Project for the Rat (NBRP-Rat) database. Genome analysis with 357 SSLP markers identified DOB/Oda-specific alleles in 70 markers. The percentage of SSLP markers that showed polymorphism between the DOB/Oda strain and any of 132 laboratory strains from NBRP-Rat varied from 89 to 95 %. The polymorphic rate (average of the values of the percentage) for the DOB/Oda strain was 91.6 %, much higher than the rates for available wild-derived strains such as the Brown Norway rat. A phylogenic tree constructed with DOB/Oda and all the strains in NBRP-Rat showed that the DOB/Oda strain localized within the wild rat groups, apparently separate from the laboratory strains. Together, these findings indicated that the DOB/Oda rat has a unique genome that is not available in the laboratory strains. Therefore, the new DOB/Oda strain will provide an important genome resource that will be useful for designing genetic experiments and for the discovery of genes that modulate mutant phenotypes.
ISSN:0938-8990
1432-1777
DOI:10.1007/s00335-013-9465-z