Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process

The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by the middle and posterior silk glands. In view of the significant role of the ASG in the liquid–crystal spinning process, a shotgun proteomics approach was taken to study the relationship between the...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2013-09, Vol.13 (17), p.2657-2663
Main Authors: Yi, Qiying, Zhao, Ping, Wang, Xin, Zou, Yong, Zhong, Xiaowu, Wang, Chen, Xiang, Zhonghuai, Xia, Qing-you
Format: Article
Language:English
Subjects:
Animal proteomics
Animals
Anterior silk gland
Bombyx - metabolism
Bombyx mori
Cuticle proteins
Exocrine Glands - chemistry
Gene Expression Profiling
Insect Proteins - analysis
Insect Proteins - chemistry
Insect Proteins - metabolism
Proteins
Proteome - analysis
Proteomics - methods
Shotgun
Silk
Silk - biosynthesis
Silk fiber
Spinning
Worms
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Summary:The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by the middle and posterior silk glands. In view of the significant role of the ASG in the liquid–crystal spinning process, a shotgun proteomics approach was taken to study the relationship between the function of proteins in the silkworm ASG and the spinning mechanism. A total of 1132 proteins with 7647 unique peptides were identified in the ASG dataset including some involved in the cuticle, ion transportation, energy metabolism, and apoptosis. Two putative cuticle‐specific proteins were highly and specifically expressed in the ASG; therefore, the ASG dataset could provide clues for comprehensive understanding of the natural silk spinning mechanism in the silkworm. All MS data have been deposited in the ProteomeXchange with identifier PXD000090.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201200443