Localized role of CRMP1 and CRMP2 in neurite outgrowth and growth cone steering

Collapsin response mediator protein 1 (CRMP1) and CRMP2 have been known as mediators of extracellular guidance cues such as semaphorin 3A and contribute to cytoskeletal reorganization in the axonal pathfinding process. To date, how CRMP1 and CRMP2 focally regulate axonal pathfinding in the growth co...

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Published in:Developmental neurobiology (Hoboken, N.J.) N.J.), 2012-12, Vol.72 (12), p.1528-1540
Main Authors: Higurashi, Masakazu, Iketani, Masumi, Takei, Kohtaro, Yamashita, Naoya, Aoki, Reina, Kawahara, Nobutaka, Goshima, Yoshio
Format: Article
Language:English
Subjects:
Animals
Axon guidance
CALI
Chick Embryo
CRMP1
CRMP2
growth cone steering
Growth Cones - metabolism
Immunoblotting
Immunohistochemistry
Intercellular Signaling Peptides and Proteins - analysis
Intercellular Signaling Peptides and Proteins - metabolism
Nerve Tissue Proteins - analysis
Nerve Tissue Proteins - metabolism
neurite outgrowth
Neurites - metabolism
Neurogenesis - physiology
Phosphoproteins - analysis
Phosphoproteins - metabolism
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Summary:Collapsin response mediator protein 1 (CRMP1) and CRMP2 have been known as mediators of extracellular guidance cues such as semaphorin 3A and contribute to cytoskeletal reorganization in the axonal pathfinding process. To date, how CRMP1 and CRMP2 focally regulate axonal pathfinding in the growth cone has not been elucidated. To delineate the local functions of these CRMPs, we carried out microscale‐chromophore‐assisted light inactivation (micro‐CALI), which enables investigation of localized molecular functions with highly spatial and temporal resolutions. Inactivation of either CRMP1 or CRMP2 in the neurite shaft led to arrested neurite outgrowth. Micro‐CALI of CRMP2 in the central domain of the growth cones consistently arrested neurite outgrowth, whereas micro‐CALI of CRMP1 in the same region caused significant lamellipodial retraction, followed by retardation of neurite outgrowth. Focal inactivation of CRMP1 in its half region of the growth cone resulted in the growth cone turning away from the irradiated site. Conversely, focal inactivation of CRMP2 resulted in the growth cone turning toward the irradiated site. These findings suggest different functions for CRMP1 and CRMP2 in growth cone behavior and neurite outgrowth. © 2012 Wiley Periodicals, Inc. Develop Neurobiol, 2012
ISSN:1932-8451
1932-846X
DOI:10.1002/dneu.22017