Transcription activator-like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing

• Published in: Biotechnology and bioengineering 2013-07, Vol.110 (7), p.1811-1821
• Main Authors: Sun, Ning, Zhao, Huimin
• Format: Article
• Language: English
• Subjects: Cells; Deoxyribonucleases - genetics; Deoxyribonucleases - metabolism; Deoxyribonucleic acid; DNA; DNA - metabolism; double-strand break; Endonucleases - genetics; Endonucleases - metabolism; Enzymes; Gene Rearrangement; gene targeting; Gene Targeting - methods; genome engineering; Genomes; Genomics - methods; homologous recombination; non-homologous end joining; Optimization; Substrate Specificity; Trans-Activators - genetics; Trans-Activators - metabolism
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.24890

Transcription activator‐like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site‐specific chromosomal double‐strand breaks introduced by TALENs significantly increase the efficiency of genomic modification. The modular nature of the TALE central repeat domains enables researchers to tailor DNA recognition specificity with ease and target essentially any desired DNA sequence. Here, we comprehensively review the development of TALEN technology in terms of scaffold optimization, DNA recognition, and repeat array assembly. In addition, we provide some perspectives on the future development of this technology. Biotechnol. Bioeng. 2013; 110: 1811–1821. © 2013 Wiley Periodicals, Inc. Transcription activator‐like effector nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. In this article, the authors comprehensively review the development of TALEN technology in terms of scaffold optimization, DNA recognition, and repeat array assembly. In addition, they provide some perspectives on the future development of this technology.