Biexponential diffusion decay in formalin-fixed prostate tissue: Preliminary findings

Magnetic resonance microimaging was used to measure diffusion decay over an extended b‐factor range in a formalin‐fixed normal prostate sample and a Gleason pattern 3+4 cancer tissue sample. The coefficients of biexponential fits to diffusion decay data from 1600 voxels of dimension 160 × 160 × 160...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2012-09, Vol.68 (3), p.954-959
Main Authors: Bourne, Roger M., Kurniawan, Nyoman, Cowin, Gary, Stait-Gardner, Timothy, Sved, Paul, Watson, Geoffrey, Chowdhury, Supriya, Price, William S.
Format: Article
Language:English
Subjects:
Algorithms
apparent diffusion coefficient
biexponential
compartmentation
Computer Simulation
Diffusion
diffusion decay
Fixatives
Formaldehyde
Humans
Image Enhancement - methods
Image Interpretation, Computer-Assisted - methods
Male
Middle Aged
Models, Biological
Pilot Projects
prostate
Prostate - pathology
Prostate - physiopathology
Prostatic Neoplasms - chemistry
Prostatic Neoplasms - pathology
Prostatic Neoplasms - physiopathology
Reproducibility of Results
Sensitivity and Specificity
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Summary:Magnetic resonance microimaging was used to measure diffusion decay over an extended b‐factor range in a formalin‐fixed normal prostate sample and a Gleason pattern 3+4 cancer tissue sample. The coefficients of biexponential fits to diffusion decay data from 1600 voxels of dimension 160 × 160 × 160 μm3 in each sample were correlated with underlying epithelial and stromal compartment partial volumes estimated from high‐resolution apparent diffusion coefficient (ADC) data (40 × 40 × 40 μm3 voxels) from the same tissue. In the normal tissue sample, the signal fractions of the low and high ADC components of the biexponential fits correlated linearly with partial volumes of epithelial tissue (R2 = 0.6) and stromal tissue (R2 = 0.5), respectively. Similar but weaker correlations were observed in the cancer sample. Epithelium‐containing high spatial resolution voxels appeared to be composed of ∼60% low ADC and ∼40% high ADC component. Stromal voxels appeared to be composed of ∼20% low ADC and ∼80% high ADC component. This preliminary report suggests that distinctly different diffusion properties in microscopically adjacent cell types contribute to the multiexponential diffusion decay phenomenon in prostate tissue. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.23291