Application of a sensitive near-field microwave microprobe to the nondestructive characterization of microbial rhodopsin

We study the opto‐electrical properties of Natronomonas pharaonis sensory rhodopsin II (NpSRII) by using a near‐field microwave microprobe (NFMM) under external light illumination. To investigate the possibility of application of NFMM to biological macromolecules, we used time dependent properties o...

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Bibliographic Details
Published in:Journal of biophotonics 2013-02, Vol.6 (2), p.163-170
Main Authors: Kim, Songhui, Yoon, Youngwoon, Lee, Hanju, Choi, Ah Reum, Jung, Kwang-Hwan, Babajanyan, Arsen, Abrahamyan, Tigran, Yoo, Hyungkeun, Lee, Jung-Ha, Cha, Deokjoon, Berthiau, Gerard, Friedman, Barry, Lee, Kiejin
Format: Article
Language:English
Subjects:
Gloeobacter
Halorhodopsins - chemistry
Halorhodopsins - radiation effects
Microwaves
Models, Theoretical
NFMM
Optical Devices
Optical Phenomena
photo-intermediate
Protein Conformation
Protein Stability
Rhodopsin - chemistry
Rhodopsin - radiation effects
Rhodopsins, Microbial - chemistry
Rhodopsins, Microbial - radiation effects
sensory rhodopsin
Sensory Rhodopsins - chemistry
Sensory Rhodopsins - radiation effects
Spectrophotometry
UV/VIS spectroscopy
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Summary:We study the opto‐electrical properties of Natronomonas pharaonis sensory rhodopsin II (NpSRII) by using a near‐field microwave microprobe (NFMM) under external light illumination. To investigate the possibility of application of NFMM to biological macromolecules, we used time dependent properties of NPSRII before/after light activation which has three distinct states – ground‐state, M‐state, and O‐state. The diagnostic ability of NFMM is demonstrated by measuring the microwave reflection coefficient (S11) spectrum of NpSRII under steady‐state illumination in the wavelength range of 350–650 nm. Moreover, we present microwave reflection coefficient S11 spectra in the same wavelength range for two fast‐photocycling rhodopsins: green light‐absorbing proteorhodopsin (GPR) and Gloeobacter rhodopsin (GR). In addition the frequency sweep shift can be detected completely even for tiny amounts of sample (∼10–3 OD of rhodopsin). Based on these results NFMM shows both very high sensitivity for detecting conformational changes and produces a good time‐resolved spectrum. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201100143