Element distribution in the corrosion layer and cytotoxicity of alloy Mg–10Dy during in vitro biodegradation

The present work investigates the corrosion behaviour, the element distribution in the corrosion layer and the cytocompatibility of alloy Mg–10Dy. The corrosion experiments were performed in a cell culture medium (CCM) under cell culture conditions close to the in vivo environment. The element distr...

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Bibliographic Details
Published in:Acta biomaterialia 2013-11, Vol.9 (10), p.8475-8487
Main Authors: Yang, Lei, Hort, Norbert, Laipple, Daniel, Höche, Daniel, Huang, Yuanding, Kainer, Karl Ulrich, Willumeit, Regine, Feyerabend, Frank
Format: Article
Language:English
Subjects:
Absorbable Implants
alloys
Alloys - pharmacology
Biocompatible Materials - pharmacology
biodegradation
calcium
cell adhesion
cell culture
Cell Death - drug effects
Cell Shape - drug effects
Cell Survival - drug effects
Corrosion
culture media
Cytocompatibility
cytotoxicity
Element distribution
Elements
energy-dispersive X-ray analysis
Humans
In vitro corrosion
Materials Testing
Mg–10Dy alloy
Microscopy, Electron, Scanning
osteoblasts
Osteoblasts - cytology
Osteoblasts - drug effects
Photoelectron Spectroscopy
scanning electron microscopy
Spectrometry, X-Ray Emission
Time Factors
X-Ray Diffraction
X-ray photoelectron spectroscopy
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Summary:The present work investigates the corrosion behaviour, the element distribution in the corrosion layer and the cytocompatibility of alloy Mg–10Dy. The corrosion experiments were performed in a cell culture medium (CCM) under cell culture conditions close to the in vivo environment. The element distribution on the surface as well as in cross-sections of the corrosion layer was investigated using scanning electron microscopy, energy-dispersive X-ray analysis, X-ray photoelectron spectroscopy and X-ray diffraction. The cytocompatibility of alloy Mg–10Dy with primary human osteoblasts was evaluated by MTT, cell adhesion and live/dead staining tests. The results show that the corrosion layer was enriched in Dy, while the P and Ca content gradually decreased from the surface to the bottom of the corrosion layer. In addition, large amounts of MgCO3·3H2O formed in the corrosion layer after 28days immersion. Both extracts and the Dy-enriched corrosion layer of alloy Mg–10Dy showed no cytotoxicity to primary human osteoblasts.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2012.10.001