The free- and protein-amino acids of the rumen phycomycete fungi Neocallimastix frontalis and Piromonas communis

The proteins and the amino acid profile of the rumen fungi Neocallimastix frontalis and Piromonas communis have been examined and the in vitro digestibility of the vegetative growth determined. The mean true protein content of N. frontalis was 24% of the dry weight and 30% for P. communis, which has...

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Bibliographic Details
Published in:The Journal of agricultural science 1985-12, Vol.105 (3), p.523-526
Main Authors: Kemp, P., Jordan, D. J., Orpin, C. G.
Format: Article
Language:English
Subjects:
amino acids
Animal, plant and microbial ecology
Biological and medical sciences
digestibility
digestion
Fundamental and applied biological sciences. Psychology
in vitro
Microbial ecology
Neocallimastix
Neocallimastix frontalis
Normal microflora of man and animals. Rumen
phycomycetes
Piromonas
protein composition
rumen
rumen fluids
rumen microorganisms
sheep
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Summary:The proteins and the amino acid profile of the rumen fungi Neocallimastix frontalis and Piromonas communis have been examined and the in vitro digestibility of the vegetative growth determined. The mean true protein content of N. frontalis was 24% of the dry weight and 30% for P. communis, which has a lower chitin content. 50% of the protein extracted from cells and examined by HPLC was in the 200000 MW range and 40% in the 50000 MW range. Polyacrylamide gel electrophoresis after treatment with sodium dodecylsulphate and 2-mercaptoethanol indicated that the native proteins were composed of smaller units in the MW range 25000–50000. The amino acid profiles of the two fungi were similar and compared favourably with those of casein and fraction 1 protein of lucerne.
ISSN:0021-8596
1469-5146
DOI:10.1017/S0021859600059402