Enhancement of Macrostome Formation in a Low-Transforming Subline of Tetrahymena vorax by Alpha-Tocopheryl Succinate and Related Compounds

The effect of alpha-tocopheryl succinate, phylloquinone, coenzyme Q9, various antioxidants, and several alcohols on microstome-macrostome transformation in a low-transforming subline of Tetrahymena vorax was investigated under non-heat shock and heat shock conditions. None of the compounds tested in...

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Bibliographic Details
Published in:Transactions of the American Microscopical Society 1985-10, Vol.104 (4), p.341-349
Main Authors: Ryals, Phillip E., Smith-Somerville, Harriett E.
Format: Article
Language:English
Subjects:
Alcohols
Antioxidants
Cell death
Coenzymes
Ethanol
Freshwater
Membrane fluidity
Shock heating
Shock tests
Solvents
Tetrahymena vorax
Vitamin E
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Summary:The effect of alpha-tocopheryl succinate, phylloquinone, coenzyme Q9, various antioxidants, and several alcohols on microstome-macrostome transformation in a low-transforming subline of Tetrahymena vorax was investigated under non-heat shock and heat shock conditions. None of the compounds tested induced morphological conversion when used without heat shock. However, when cells of the microstomal form were first subjected to heat shocks and then washed into certain test solutions, a significant increase in the percentage of macrostomal cells produced was observed. Alpha-tocopheryl succinate (10-4M), phylloquinone (10-4M), coenzyme Q9(10-4M), and 0.095% 1-pentanol were the most effective enhancers of heat shock-induced macrostome production. The alcohols 1-propanol (0.95%), 1-butanol (0.095%), and 2-pentanol (0.095%) also increased the yield of macrostomal cells formed following heat shock, but to a lesser degree. Although alpha-tocopheryl succinate is known to possess antioxidant properties, other antioxidants tested produced no significant enhancement of macrostomal cell formation. This suggests that such enhancement by alpha-tocopheryl succinate is accomplished by means of an as yet unknown mechanism.
ISSN:0003-0023
DOI:10.2307/3226487