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Inhibitory effect of upregulated DR-nm23 expression on invasion and metastasis in colorectal cancer

To explore the role of DR-nm23 in the regulation of colorectal cancer invasion and metastasis. Immunohistochemistry assay and in-situ nucleic acid hybridization were carried out to analyze the protein and mRNA expression of DR-nm23 in patient samples. The molecular cloning technique was applied to c...

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Published in:European journal of cancer prevention 2013-11, Vol.22 (6), p.512-522
Main Authors: Qu, Lijuan, Liang, Li, Su, Juanjuan, Yang, Zhi
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Su, Juanjuan
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description To explore the role of DR-nm23 in the regulation of colorectal cancer invasion and metastasis. Immunohistochemistry assay and in-situ nucleic acid hybridization were carried out to analyze the protein and mRNA expression of DR-nm23 in patient samples. The molecular cloning technique was applied to construct the recombinant lentiviral expression vector pGC-FU-DRnm23-GFP . Lentiviral infection was used to introduce overexpression of exogenous DR-nm23 in SW620 colorectal cancer cells. Both in-vitro cell experiments and an in-vivo xenograft tumor model assay were carried out to determine the role of DR-nm23 in the regulation of colorectal cancer proliferation, invasion, and metastasis. Our data here showed that the expression level of DR-nm23 in colorectal cancer tissue was significantly lower than that in adenoma and normal tissue. The expression level of DR-nm23 was also found to be negatively correlated with lymph node metastasis and positively correlated with the degree of tumor differentiation. Besides, introduced overexpression of DR-nm23 in SW620 cells through lentiviral infection resulted in significant inhibition of its proliferation rate in vitro and growth rate in vivo. The cell migration ability in vitro and metastatic potential in vivo were also impaired at the same time. Our current study suggested that DR-nm23 may participate in the regulation of differentiation of colorectal cancer cells. Its downregulated expression is closely related to the invasion and metastasis of colorectal cancer. Thus, expression status of DR-nm23 may act as a potential prognostic factor in patients with colorectal cancer.
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Immunohistochemistry assay and in-situ nucleic acid hybridization were carried out to analyze the protein and mRNA expression of DR-nm23 in patient samples. The molecular cloning technique was applied to construct the recombinant lentiviral expression vector pGC-FU-DRnm23-GFP . Lentiviral infection was used to introduce overexpression of exogenous DR-nm23 in SW620 colorectal cancer cells. Both in-vitro cell experiments and an in-vivo xenograft tumor model assay were carried out to determine the role of DR-nm23 in the regulation of colorectal cancer proliferation, invasion, and metastasis. Our data here showed that the expression level of DR-nm23 in colorectal cancer tissue was significantly lower than that in adenoma and normal tissue. The expression level of DR-nm23 was also found to be negatively correlated with lymph node metastasis and positively correlated with the degree of tumor differentiation. Besides, introduced overexpression of DR-nm23 in SW620 cells through lentiviral infection resulted in significant inhibition of its proliferation rate in vitro and growth rate in vivo. The cell migration ability in vitro and metastatic potential in vivo were also impaired at the same time. Our current study suggested that DR-nm23 may participate in the regulation of differentiation of colorectal cancer cells. Its downregulated expression is closely related to the invasion and metastasis of colorectal cancer. Thus, expression status of DR-nm23 may act as a potential prognostic factor in patients with colorectal cancer.</description><identifier>ISSN: 0959-8278</identifier><identifier>EISSN: 1473-5709</identifier><identifier>DOI: 10.1097/CEJ.0b013e328361625d</identifier><identifier>PMID: 23765094</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams &amp; Wilkins, a business of Wolters Kluwer Health</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - prevention &amp; control ; Adenocarcinoma - secondary ; Adenoma - genetics ; Adenoma - pathology ; Adenoma - prevention &amp; control ; Adult ; Aged ; Animals ; Apoptosis ; Biological and medical sciences ; Blotting, Western ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Colon - metabolism ; Colon - pathology ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - prevention &amp; control ; Female ; Gene Expression Regulation, Neoplastic - physiology ; Humans ; Immunoenzyme Techniques ; Lentivirus - genetics ; Lymphatic Metastasis ; Male ; Medical sciences ; Mice ; Mice, Nude ; Middle Aged ; Miscellaneous ; Multiple tumors. Solid tumors. Tumors in childhood (general aspects) ; Neoplasm Grading ; Neoplasm Invasiveness ; NM23 Nucleoside Diphosphate Kinases - genetics ; Prevention and actions ; Public health. Hygiene ; Public health. Hygiene-occupational medicine ; Real-Time Polymerase Chain Reaction ; Rectum - metabolism ; Rectum - pathology ; Research paper ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Tumor Cells, Cultured ; Tumors ; Up-Regulation</subject><ispartof>European journal of cancer prevention, 2013-11, Vol.22 (6), p.512-522</ispartof><rights>2013 Wolters Kluwer Health | Lippincott Williams &amp; Wilkins</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-89441b2a2a89b94b9cc4877d48fb994d50ac41ffd636eaa8a731248c9cb4ff583</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48504218$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48504218$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=27854036$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23765094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qu, Lijuan</creatorcontrib><creatorcontrib>Liang, Li</creatorcontrib><creatorcontrib>Su, Juanjuan</creatorcontrib><creatorcontrib>Yang, Zhi</creatorcontrib><title>Inhibitory effect of upregulated DR-nm23 expression on invasion and metastasis in colorectal cancer</title><title>European journal of cancer prevention</title><addtitle>Eur J Cancer Prev</addtitle><description>To explore the role of DR-nm23 in the regulation of colorectal cancer invasion and metastasis. Immunohistochemistry assay and in-situ nucleic acid hybridization were carried out to analyze the protein and mRNA expression of DR-nm23 in patient samples. The molecular cloning technique was applied to construct the recombinant lentiviral expression vector pGC-FU-DRnm23-GFP . Lentiviral infection was used to introduce overexpression of exogenous DR-nm23 in SW620 colorectal cancer cells. Both in-vitro cell experiments and an in-vivo xenograft tumor model assay were carried out to determine the role of DR-nm23 in the regulation of colorectal cancer proliferation, invasion, and metastasis. Our data here showed that the expression level of DR-nm23 in colorectal cancer tissue was significantly lower than that in adenoma and normal tissue. The expression level of DR-nm23 was also found to be negatively correlated with lymph node metastasis and positively correlated with the degree of tumor differentiation. Besides, introduced overexpression of DR-nm23 in SW620 cells through lentiviral infection resulted in significant inhibition of its proliferation rate in vitro and growth rate in vivo. The cell migration ability in vitro and metastatic potential in vivo were also impaired at the same time. Our current study suggested that DR-nm23 may participate in the regulation of differentiation of colorectal cancer cells. Its downregulated expression is closely related to the invasion and metastasis of colorectal cancer. Thus, expression status of DR-nm23 may act as a potential prognostic factor in patients with colorectal cancer.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - prevention &amp; control</subject><subject>Adenocarcinoma - secondary</subject><subject>Adenoma - genetics</subject><subject>Adenoma - pathology</subject><subject>Adenoma - prevention &amp; control</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - prevention &amp; control</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Lentivirus - genetics</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Invasiveness</subject><subject>NM23 Nucleoside Diphosphate Kinases - genetics</subject><subject>Prevention and actions</subject><subject>Public health. Hygiene</subject><subject>Public health. Hygiene-occupational medicine</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Rectum - metabolism</subject><subject>Rectum - pathology</subject><subject>Research paper</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Up-Regulation</subject><issn>0959-8278</issn><issn>1473-5709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpdkFtLHTEQgIO06Kn6D7TkpdCXtblfHsupthahUPR5mWQTXdndnCa7Rf-90XOqUBhImPlmhvkQOqHkjBKrv6zPf54RRygPnBmuqGKy20MrKjRvpCb2HVoRK21jmDYH6EMp94RQzanaRweMayWJFSvkL6e73vVzyo84xBj8jFPEyyaH22WAOXT42-9mGhnH4aEmS-nThGv00194-cPU4THMUGr0peaxT0PKdRAM2MPkQz5C7yMMJRzv3kN0c3F-vf7RXP36frn-etV4Lu3cGCsEdQwYGOuscNZ7YbTuhInOWtFJAl7QGDvFVQAwUI9hwnjrnYhRGn6IPm_nbnL6s4Qyt2NffBgGmEJaSksFV1Jxq1hFxRb1OZWSQ2w3uR8hP7aUtM9626q3_V9vbfu427C4MXSvTf98VuDTDoDiYYi5CujLG6eNFISryp1uuftS1b_WhZFEMGr4E5fqjf0</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Qu, Lijuan</creator><creator>Liang, Li</creator><creator>Su, Juanjuan</creator><creator>Yang, Zhi</creator><general>Lippincott Williams &amp; Wilkins, a business of Wolters Kluwer Health</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131101</creationdate><title>Inhibitory effect of upregulated DR-nm23 expression on invasion and metastasis in colorectal cancer</title><author>Qu, Lijuan ; Liang, Li ; Su, Juanjuan ; Yang, Zhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-89441b2a2a89b94b9cc4877d48fb994d50ac41ffd636eaa8a731248c9cb4ff583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - prevention &amp; control</topic><topic>Adenocarcinoma - secondary</topic><topic>Adenoma - genetics</topic><topic>Adenoma - pathology</topic><topic>Adenoma - prevention &amp; control</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - prevention &amp; control</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic - physiology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Lentivirus - genetics</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Invasiveness</topic><topic>NM23 Nucleoside Diphosphate Kinases - genetics</topic><topic>Prevention and actions</topic><topic>Public health. Hygiene</topic><topic>Public health. Hygiene-occupational medicine</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Rectum - metabolism</topic><topic>Rectum - pathology</topic><topic>Research paper</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qu, Lijuan</creatorcontrib><creatorcontrib>Liang, Li</creatorcontrib><creatorcontrib>Su, Juanjuan</creatorcontrib><creatorcontrib>Yang, Zhi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cancer prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qu, Lijuan</au><au>Liang, Li</au><au>Su, Juanjuan</au><au>Yang, Zhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of upregulated DR-nm23 expression on invasion and metastasis in colorectal cancer</atitle><jtitle>European journal of cancer prevention</jtitle><addtitle>Eur J Cancer Prev</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>22</volume><issue>6</issue><spage>512</spage><epage>522</epage><pages>512-522</pages><issn>0959-8278</issn><eissn>1473-5709</eissn><abstract>To explore the role of DR-nm23 in the regulation of colorectal cancer invasion and metastasis. Immunohistochemistry assay and in-situ nucleic acid hybridization were carried out to analyze the protein and mRNA expression of DR-nm23 in patient samples. The molecular cloning technique was applied to construct the recombinant lentiviral expression vector pGC-FU-DRnm23-GFP . Lentiviral infection was used to introduce overexpression of exogenous DR-nm23 in SW620 colorectal cancer cells. Both in-vitro cell experiments and an in-vivo xenograft tumor model assay were carried out to determine the role of DR-nm23 in the regulation of colorectal cancer proliferation, invasion, and metastasis. Our data here showed that the expression level of DR-nm23 in colorectal cancer tissue was significantly lower than that in adenoma and normal tissue. The expression level of DR-nm23 was also found to be negatively correlated with lymph node metastasis and positively correlated with the degree of tumor differentiation. Besides, introduced overexpression of DR-nm23 in SW620 cells through lentiviral infection resulted in significant inhibition of its proliferation rate in vitro and growth rate in vivo. The cell migration ability in vitro and metastatic potential in vivo were also impaired at the same time. Our current study suggested that DR-nm23 may participate in the regulation of differentiation of colorectal cancer cells. Its downregulated expression is closely related to the invasion and metastasis of colorectal cancer. Thus, expression status of DR-nm23 may act as a potential prognostic factor in patients with colorectal cancer.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams &amp; Wilkins, a business of Wolters Kluwer Health</pub><pmid>23765094</pmid><doi>10.1097/CEJ.0b013e328361625d</doi><tpages>11</tpages></addata></record>
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identifier ISSN: 0959-8278
ispartof European journal of cancer prevention, 2013-11, Vol.22 (6), p.512-522
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source JSTOR Archival Journals and Primary Sources Collection
subjects Adenocarcinoma - genetics
Adenocarcinoma - prevention & control
Adenocarcinoma - secondary
Adenoma - genetics
Adenoma - pathology
Adenoma - prevention & control
Adult
Aged
Animals
Apoptosis
Biological and medical sciences
Blotting, Western
Cell Adhesion
Cell Movement
Cell Proliferation
Colon - metabolism
Colon - pathology
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Colorectal Neoplasms - prevention & control
Female
Gene Expression Regulation, Neoplastic - physiology
Humans
Immunoenzyme Techniques
Lentivirus - genetics
Lymphatic Metastasis
Male
Medical sciences
Mice
Mice, Nude
Middle Aged
Miscellaneous
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Neoplasm Grading
Neoplasm Invasiveness
NM23 Nucleoside Diphosphate Kinases - genetics
Prevention and actions
Public health. Hygiene
Public health. Hygiene-occupational medicine
Real-Time Polymerase Chain Reaction
Rectum - metabolism
Rectum - pathology
Research paper
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Tumor Cells, Cultured
Tumors
Up-Regulation
title Inhibitory effect of upregulated DR-nm23 expression on invasion and metastasis in colorectal cancer
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