MEK1/2 differentially participates in GnRH actions on goldfish LH and GH secretion and hormone protein availability: Acute and long-term effects, in vitro

• Published in: General and comparative endocrinology 2013-10, Vol.192, p.149-158
• Main Authors: Pemberton, Joshua G, Orr, Michael E, Booth, Morgan, Chang, John P
• Format: Article
• Language: English
• Subjects: Animals; Butadienes - pharmacology; Endocrinology & Metabolism; Flavonoids - pharmacology; goldfish; Goldfish - metabolism; gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - metabolism; Growth Hormone - genetics; Growth Hormone - metabolism; hormone secretion; long term effects; luteinizing hormone; Luteinizing Hormone - genetics; Luteinizing Hormone - metabolism; MAP Kinase Kinase 1 - genetics; MAP Kinase Kinase 1 - metabolism; MAP Kinase Kinase 2 - genetics; MAP Kinase Kinase 2 - metabolism; MEK1/2 and ERK1/2 signaling; mitogen-activated protein kinase; Nitriles - pharmacology; Perifusion and static incubation; PKC signaling; Primary cultures of dispersed goldfish pituitary cells; protein kinase C; signal transduction; Signal Transduction - drug effects; U0126 and PD098059
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2013.03.017

Highlights • First time MEK1/2 involvement in GnRH actions on GH release has been investigated. • Differential involvement of MEK1/2 signaling in PKC-stimulated LH and GH release. • Temporal and GnRH-isoform differences in MEK1/2 involvement in LH and GH release. • Differential effects of MEK1/2 inhibition on basal LH and GH protein availability. • Dissociation of GnRH effects on hormone mRNA expression and protein content.