Increased Nucleic Acid Receptor Expression in Chronic Periodontitis

Background: Nucleic acid sensing has emerged as one of the important components of the immune system triggering inflammation. The aim of this study is to determine the expression of bacterial DNA sensors, including Toll‐like receptor 9 (TLR‐9), DNA‐dependent activator of interferon‐regulatory factor...

Full description

Saved in:
Bibliographic Details
Published in:Journal of periodontology (1970) 2013-10, Vol.84 (10), p.e48-e57
Main Authors: Sahingur, S. Esra, Xia, Xia‐Juan, Voth, Stephanie C., Yeudall, W. Andrew, Gunsolley, John C.
Format: Article
Language:English
Subjects:
Adult
Aged
AIM2
Chronic Periodontitis - immunology
Dentistry
DNA, bacterial
DNA-Binding Proteins - analysis
Female
Gingiva - immunology
Humans
inflammation
Male
Melanoma - immunology
Middle Aged
Nuclear Proteins - analysis
periodontal disease
periodontitis
Periodontium - immunology
Real-Time Polymerase Chain Reaction
RNA, Messenger - analysis
Toll-Like Receptor 1 - analysis
Toll-Like Receptor 2 - analysis
Toll-Like Receptor 3 - analysis
Toll-Like Receptor 4 - analysis
Toll-Like Receptor 5 - analysis
Toll-Like Receptor 6 - analysis
Toll-Like Receptor 7 - analysis
Toll-Like Receptor 8 - analysis
Toll-Like Receptor 9 - analysis
Toll-Like Receptors - analysis
Toll‐like receptors
Up-Regulation
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Nucleic acid sensing has emerged as one of the important components of the immune system triggering inflammation. The aim of this study is to determine the expression of bacterial DNA sensors, including Toll‐like receptor 9 (TLR‐9), DNA‐dependent activator of interferon‐regulatory factors (DAI), and absent in melanoma 2 (AIM2) in chronic periodontitis (CP versus healthy) (H) tissues. Methods: Thirty‐five CP and 27 H gingival biopsies were included. Real‐time quantitative polymerase chain reaction was performed to determine mRNA levels of AIM2, DAI, and TLRs (TLR‐1 through TLR‐9). The difference in gene expression for each sensor between CP and H tissues was calculated using analysis of covariance. The Spearman test was used to determine correlations among innate receptors. The expression of TLR‐9, AIM2, and DAI in gingival tissues was further confirmed using immunohistochemistry. Results: The present results reveal statistically significant upregulation of TLR‐9 (P
ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2013.120739