The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion

Abstract Objective To investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion. Study design Amnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 s...

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Bibliographic Details
Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2013-09, Vol.89 (4), p.221-225
Main Authors: Frew, L, Sugiarto, N.U, Rajagopal, S.P, He, J, Leask, R, Norman, J.E, Riley, S.C, Stock, S.J
Format: Article
Language:English
Subjects:
Advanced Basic Science
Amnion - drug effects
Amnion - immunology
Amnion - metabolism
Anilides - pharmacology
Anti-Inflammatory Agents - pharmacology
Chromans - pharmacology
Cytokines - metabolism
Drug Evaluation, Preclinical
Endocrinology & Metabolism
Fatty Acids, Omega-3 - pharmacology
Female
Foetal membranes
Humans
Inflammation
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - metabolism
Parturition
Polyunsaturated fatty acids
PPAR gamma - antagonists & inhibitors
PPAR gamma - metabolism
PPARγ
Pregnancy
Thiazolidinediones - pharmacology
Tissue Culture Techniques
Troglitazone
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Summary:Abstract Objective To investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion. Study design Amnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography. Results A combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls ( p
ISSN:0952-3278
1532-2823
DOI:10.1016/j.plefa.2013.07.001