Matrix-Assisted Laser Desorption and Ionization-Time-of-Flight Mass Spectrometry, 16S rRNA Gene Sequencing, and API 32E for Identification of Cronobacter spp.: A Comparative Study

Twenty-two isolates of the family Enterobacteriaceae, with focus on Cronobacter isolated from infant formula and the environment of milk powder plants, were comparatively identified using API 32E (bioMérieux, Marcy l'Etoile, France), 16S rRNA gene sequencing (Accugenix, Newark, USA), and matrix...

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Bibliographic Details
Published in:Journal of food protection 2011-12, Vol.74 (12), p.2182-2187
Main Authors: SHA ZHU, RATERING, Stefan, SCHNELL, Sylvia, WACKER, Ron
Format: Article
Language:English
Subjects:
Baby foods
Biological and medical sciences
Biotechnology
Comparative studies
Cronobacter - classification
Cronobacter - isolation & purification
Desorption
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Enterobacteriaceae
Food Contamination - analysis
Food engineering
Food industries
Food microbiology
Food safety
Fundamental and applied biological sciences. Psychology
General aspects
Humans
Identification systems
Infant
Infant Food - microbiology
Ionization
Ions
Lasers
Manufacturers
Mass spectrometry
Pathogens
Phylogenetics
Phylogeny
Proteins
Ribonucleic acid
Risk assessment
RNA
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Scientific imaging
Sequence Analysis, DNA
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Taxonomy
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Summary:Twenty-two isolates of the family Enterobacteriaceae, with focus on Cronobacter isolated from infant formula and the environment of milk powder plants, were comparatively identified using API 32E (bioMérieux, Marcy l'Etoile, France), 16S rRNA gene sequencing (Accugenix, Newark, USA), and matrix-assisted laser desorption and ionization-time-of-flight mass spectrometry (MALDI-TOF MS; Mabritec, Riehen, Switzerland and AnagnosTec, Potsdam, Germany). With API 32E, 22% of the isolates were assigned to species, 64% were assigned to a genus, and 14% could not be discriminated at any taxonomic level. Both 16S rRNA gene sequencing and MALDI-TOF MS assigned 100% of the isolates to species, but the identifications based on MALDI-TOF MS results were more discriminating and unequivocal. Our data indicate that MALDI-TOF MS provides the most rapid and unambiguous identification of Cronobacter and closely related Enterobacteriaceae isolates.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X.JFP-11-205