A Molecular Beacon-Based Signal-Off Surface-Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection

Stem‐loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon‐based signal‐off surface‐ enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle‐decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a co...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2013-08, Vol.9 (15), p.2493-2499
Main Authors: Wei, Xinpan, Su, Shao, Guo, Yuanyuan, Jiang, Xiangxu, Zhong, Yiling, Su, Yuanyuan, Fan, Chunhai, Lee, Shuit-Tong, He, Yao
Format: Article
Language:English
Subjects:
DNA
DNA - analysis
Gold - chemistry
molecular beacons
Molecular Probe Techniques
Molecular Probes - metabolism
Nanoparticles - ultrastructure
Nanotechnology
nanowires
oligonucleotides
Reproducibility of Results
Spectrum Analysis, Raman
Surface Properties
surface-enhanced Raman scattering
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Summary:Stem‐loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon‐based signal‐off surface‐ enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle‐decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a concentration down to ≈10 fM is detected under irradiation from a low‐power laser, which is better than or comparable to that detected by signal‐on SERS methods. The signal‐off strategy allows identification of single‐base mismatches and detection of multiplexed DNA.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201202914