Changes in the Level of Spontaneous DNA Damage in Whole Blood Leukocytes during Storage

We evaluated structural damage to DNA (%TDNA) in blood leukocytes from healthy donors of different age at different periods (0-6 days) of blood storage at 4-8°C. It was found that the basal level of DNA damage increased and intracellular antioxidant level decreased during storage. Mean %TDNA was 6.8...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2012-11, Vol.154 (1), p.37-39
Main Authors: Sirota, N. P., Kuznetsova, E. A.
Format: Article
Language:English
Subjects:
Adult
Aged
Antioxidants
Antioxidants - analysis
Biomedical and Life Sciences
Biomedicine
Blood Specimen Collection
Cell Biology
DNA
DNA - blood
DNA Damage
Humans
Internal Medicine
Laboratory Medicine
Leukocytes - chemistry
Middle Aged
Pathology
Reactive Oxygen Species
Refrigeration
Time Factors
Young Adult
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Summary:We evaluated structural damage to DNA (%TDNA) in blood leukocytes from healthy donors of different age at different periods (0-6 days) of blood storage at 4-8°C. It was found that the basal level of DNA damage increased and intracellular antioxidant level decreased during storage. Mean %TDNA was 6.8±3.3% in the fresh blood and 19.2±8.1% after 5-day storage. The experiments with exposure to reactive oxygen species induced by irradiation suggest that depletion of low-molecular-weight endogenous antioxidants occurs as soon as after 5-h storage. Our results suggest that storage time should be taken into account when assessing the basal and induced levels of leukocyte DNA damage by the comet assay.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-012-1869-3