Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family Potyviridae

• Published in: Archives of virology 2013-09, Vol.158 (9), p.1917-1923
• Main Authors: Mollov, Dimitre, Lockhart, Ben, Zlesak, David
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Biomedical and Life Sciences; Biomedicine; Capsids; Cistrons; Coat protein; Genome, Viral - genetics; Infectious Diseases; Medical Microbiology; Nucleotide sequence; Original Article; Phylogeny; Plant Diseases - virology; Plant viruses; Potyviridae; Potyviridae - classification; Potyviridae - genetics; RNA, Viral - genetics; Rosa - virology; Sequence analysis; Sequence Analysis, DNA; Viral Proteins - chemistry; Viral Proteins - genetics; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-013-1686-7

The complete genomic sequence of rose yellow mosaic virus (RoYMV) was determined and found to have all the features that are characteristic of members of the family Potyviridae . The RoYMV genome is 9508 nucleotides long excluding the 3′-poly-(A) tail and contains a single open reading frame encoding a polyprotein of 3067 amino acids. The RoYMV P3 and CI cistrons are shorter than those of other members of the family Potyviridae , and the 6K1 cistron is completely absent. Comparative sequence analysis revealed that RoYMV had highest amino acid sequence identity across the entire genome sequence to brome streak mosaic virus (33 %) and to turnip mosaic virus (30 %) at the coat protein level. Based on its low sequence similarity to known members of the family Potyviridae and phylogenetic analysis, RoYMV appears to be a distinct, previously undescribed, member of this family.