Loading…
Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge
The objective of the study was to investigate an association between serum cortisol and progesterone (P 4 ) concentrations and the distribution of immune cells in the endometrium of the gilts with vaginal discharge. Genital organs from 39 Landrace×Yorkshire crossbred gilts culled owing to vaginal di...
Saved in:
| Published in: | Comparative clinical pathology 2011-12, Vol.20 (6), p.563-571 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023 |
| container_end_page | 571 |
| container_issue | 6 |
| container_start_page | 563 |
| container_title | Comparative clinical pathology |
| container_volume | 20 |
| creator | Roongsitthichai, Atthaporn Suwimonteerabutr, Junpen Kaeoket, Kampon Koonjaenak, Seri Tummaruk, Padet |
| description | The objective of the study was to investigate an association between serum cortisol and progesterone (P
4
) concentrations and the distribution of immune cells in the endometrium of the gilts with vaginal discharge. Genital organs from 39 Landrace×Yorkshire crossbred gilts culled owing to vaginal discharge problem were collected from two commercial swine herds in Thailand. The estrous stage and gross pathology were examined. Blood samples were collected from the jugular vein prior to being slaughtered. Serum P
4
and cortisol were analyzed by means of enzyme immunoassay. The samples observed were in inactive (
n
= 4), follicular (
n
= 10), and luteal (
n
= 25) phases. They, afterwards, were processed in hematoxylin and eosin sections. The endometrium of the gilts was histologically divided into three layers, i.e., epithelial, subepithelial connective tissue, and glandular connective tissue layers. Immune cells, i.e., lymphocytes, neutrophils, eosinophils, macrophages, and plasma cells, in each layer were quantified under a light microscope (×400). The results revealed that mean serum cortisol was 430.6 ± 68.3 nmol/l. Serum P
4
varied by ovarian status. Serum P
4
of the gilts in the luteal phase was higher than those in the follicular phase (88.3 ± 7.7 versus 20.6 ± 6.2 nmol/l,
P |
| doi_str_mv | 10.1007/s00580-010-1033-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1439235712</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1439235712</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023</originalsourceid><addsrcrecordid>eNp1kc1q3DAUhU1poOkkD9CdoJtu3Fz92ZplCElbCGTTQnZGlq89Gmxpqmsn5FX6tNXMhBACWennfOfqXN2i-MLhOweoLwhAGyiBQ8lBylJ8KE55xU2pK33_8WWv-KfiM9EWgGsj5Wnx75IoOm9nHwNrcX5EDIwwLRNzMc2e4shs6NguxQFpxhQDZiU4DHM6uOigzxtkPvR-fL5lsWd-mpY9jeNIWTwwGLo44Zx8fiAjQzYQe_Tzhj3YwQc7ss6T29g04Flx0tuR8Px5XRV_bq5_X_0sb-9-_Lq6vC2dqJQoO6Mlt2glcqO5M1ZVrVYt1L10olZ1i3Ld1b3uVGeEctL01oG0qrXQgwQhV8W3Y93c498lN9lMOUIObQPGhRqu5FpIXfM9-vUNuo1LyqkzBWtdCZM_NlP8SLkUiRL2zS75yaanDDX7aTXHaTVwOEvZ7CuLo4cyGwZMryu_Z_oPGeWayA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1095628001</pqid></control><display><type>article</type><title>Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge</title><source>Springer Nature</source><creator>Roongsitthichai, Atthaporn ; Suwimonteerabutr, Junpen ; Kaeoket, Kampon ; Koonjaenak, Seri ; Tummaruk, Padet</creator><creatorcontrib>Roongsitthichai, Atthaporn ; Suwimonteerabutr, Junpen ; Kaeoket, Kampon ; Koonjaenak, Seri ; Tummaruk, Padet</creatorcontrib><description>The objective of the study was to investigate an association between serum cortisol and progesterone (P
4
) concentrations and the distribution of immune cells in the endometrium of the gilts with vaginal discharge. Genital organs from 39 Landrace×Yorkshire crossbred gilts culled owing to vaginal discharge problem were collected from two commercial swine herds in Thailand. The estrous stage and gross pathology were examined. Blood samples were collected from the jugular vein prior to being slaughtered. Serum P
4
and cortisol were analyzed by means of enzyme immunoassay. The samples observed were in inactive (
n
= 4), follicular (
n
= 10), and luteal (
n
= 25) phases. They, afterwards, were processed in hematoxylin and eosin sections. The endometrium of the gilts was histologically divided into three layers, i.e., epithelial, subepithelial connective tissue, and glandular connective tissue layers. Immune cells, i.e., lymphocytes, neutrophils, eosinophils, macrophages, and plasma cells, in each layer were quantified under a light microscope (×400). The results revealed that mean serum cortisol was 430.6 ± 68.3 nmol/l. Serum P
4
varied by ovarian status. Serum P
4
of the gilts in the luteal phase was higher than those in the follicular phase (88.3 ± 7.7 versus 20.6 ± 6.2 nmol/l,
P <
0.05). As for the endometrium condition, the gilts were classified into acute/subacute endometritis (
n
= 13), chronic endometritis (
n
= 9), and normal endometrium (
n
= 17). Neutrophils were the main local immune cells in the epithelial layer. Lymphocytes were the dominant population in the subepithelial and glandular connective tissue layers. Generally, the serum cortisol tended to negatively correlate with lymphocytes in the subepithelial connective tissue layer (
r
= −0.28,
P =
0.081). In the gilts with acute/subacute endometritis, no correlation among serum cortisol, P
4
, and immune cells was observed. In chronic endometritis gilts, only a negative correlation was remarked between P
4
and epithelial lymphocytes (
r
= −0.83,
P =
0.010), epithelial neutrophils (
r
= −0.79,
P =
0.019), and subepithelial neutrophils (
r
= −0.73,
P =
0.025). In the gilts with normal endometrium, P
4
negatively correlated with subepithelial neutrophils (
r
= −0.55,
P =
0.022) while positively correlated with subepithelial macrophages (
r
= 0.54,
P =
0.024) and subepithelial eosinophils (
r
= 0.60,
P =
0.011).</description><identifier>ISSN: 1618-5641</identifier><identifier>EISSN: 1618-565X</identifier><identifier>DOI: 10.1007/s00580-010-1033-2</identifier><language>eng</language><publisher>London: Springer-Verlag</publisher><subject>Hematology ; Medicine ; Medicine & Public Health ; Oncology ; Original Article ; Pathology</subject><ispartof>Comparative clinical pathology, 2011-12, Vol.20 (6), p.563-571</ispartof><rights>Springer-Verlag London Limited 2010</rights><rights>Springer-Verlag London Limited 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023</citedby><cites>FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Roongsitthichai, Atthaporn</creatorcontrib><creatorcontrib>Suwimonteerabutr, Junpen</creatorcontrib><creatorcontrib>Kaeoket, Kampon</creatorcontrib><creatorcontrib>Koonjaenak, Seri</creatorcontrib><creatorcontrib>Tummaruk, Padet</creatorcontrib><title>Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge</title><title>Comparative clinical pathology</title><addtitle>Comp Clin Pathol</addtitle><description>The objective of the study was to investigate an association between serum cortisol and progesterone (P
4
) concentrations and the distribution of immune cells in the endometrium of the gilts with vaginal discharge. Genital organs from 39 Landrace×Yorkshire crossbred gilts culled owing to vaginal discharge problem were collected from two commercial swine herds in Thailand. The estrous stage and gross pathology were examined. Blood samples were collected from the jugular vein prior to being slaughtered. Serum P
4
and cortisol were analyzed by means of enzyme immunoassay. The samples observed were in inactive (
n
= 4), follicular (
n
= 10), and luteal (
n
= 25) phases. They, afterwards, were processed in hematoxylin and eosin sections. The endometrium of the gilts was histologically divided into three layers, i.e., epithelial, subepithelial connective tissue, and glandular connective tissue layers. Immune cells, i.e., lymphocytes, neutrophils, eosinophils, macrophages, and plasma cells, in each layer were quantified under a light microscope (×400). The results revealed that mean serum cortisol was 430.6 ± 68.3 nmol/l. Serum P
4
varied by ovarian status. Serum P
4
of the gilts in the luteal phase was higher than those in the follicular phase (88.3 ± 7.7 versus 20.6 ± 6.2 nmol/l,
P <
0.05). As for the endometrium condition, the gilts were classified into acute/subacute endometritis (
n
= 13), chronic endometritis (
n
= 9), and normal endometrium (
n
= 17). Neutrophils were the main local immune cells in the epithelial layer. Lymphocytes were the dominant population in the subepithelial and glandular connective tissue layers. Generally, the serum cortisol tended to negatively correlate with lymphocytes in the subepithelial connective tissue layer (
r
= −0.28,
P =
0.081). In the gilts with acute/subacute endometritis, no correlation among serum cortisol, P
4
, and immune cells was observed. In chronic endometritis gilts, only a negative correlation was remarked between P
4
and epithelial lymphocytes (
r
= −0.83,
P =
0.010), epithelial neutrophils (
r
= −0.79,
P =
0.019), and subepithelial neutrophils (
r
= −0.73,
P =
0.025). In the gilts with normal endometrium, P
4
negatively correlated with subepithelial neutrophils (
r
= −0.55,
P =
0.022) while positively correlated with subepithelial macrophages (
r
= 0.54,
P =
0.024) and subepithelial eosinophils (
r
= 0.60,
P =
0.011).</description><subject>Hematology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Pathology</subject><issn>1618-5641</issn><issn>1618-565X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp1kc1q3DAUhU1poOkkD9CdoJtu3Fz92ZplCElbCGTTQnZGlq89Gmxpqmsn5FX6tNXMhBACWennfOfqXN2i-MLhOweoLwhAGyiBQ8lBylJ8KE55xU2pK33_8WWv-KfiM9EWgGsj5Wnx75IoOm9nHwNrcX5EDIwwLRNzMc2e4shs6NguxQFpxhQDZiU4DHM6uOigzxtkPvR-fL5lsWd-mpY9jeNIWTwwGLo44Zx8fiAjQzYQe_Tzhj3YwQc7ss6T29g04Flx0tuR8Px5XRV_bq5_X_0sb-9-_Lq6vC2dqJQoO6Mlt2glcqO5M1ZVrVYt1L10olZ1i3Ld1b3uVGeEctL01oG0qrXQgwQhV8W3Y93c498lN9lMOUIObQPGhRqu5FpIXfM9-vUNuo1LyqkzBWtdCZM_NlP8SLkUiRL2zS75yaanDDX7aTXHaTVwOEvZ7CuLo4cyGwZMryu_Z_oPGeWayA</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Roongsitthichai, Atthaporn</creator><creator>Suwimonteerabutr, Junpen</creator><creator>Kaeoket, Kampon</creator><creator>Koonjaenak, Seri</creator><creator>Tummaruk, Padet</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>201112</creationdate><title>Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge</title><author>Roongsitthichai, Atthaporn ; Suwimonteerabutr, Junpen ; Kaeoket, Kampon ; Koonjaenak, Seri ; Tummaruk, Padet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Hematology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roongsitthichai, Atthaporn</creatorcontrib><creatorcontrib>Suwimonteerabutr, Junpen</creatorcontrib><creatorcontrib>Kaeoket, Kampon</creatorcontrib><creatorcontrib>Koonjaenak, Seri</creatorcontrib><creatorcontrib>Tummaruk, Padet</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Comparative clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roongsitthichai, Atthaporn</au><au>Suwimonteerabutr, Junpen</au><au>Kaeoket, Kampon</au><au>Koonjaenak, Seri</au><au>Tummaruk, Padet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge</atitle><jtitle>Comparative clinical pathology</jtitle><stitle>Comp Clin Pathol</stitle><date>2011-12</date><risdate>2011</risdate><volume>20</volume><issue>6</issue><spage>563</spage><epage>571</epage><pages>563-571</pages><issn>1618-5641</issn><eissn>1618-565X</eissn><abstract>The objective of the study was to investigate an association between serum cortisol and progesterone (P
4
) concentrations and the distribution of immune cells in the endometrium of the gilts with vaginal discharge. Genital organs from 39 Landrace×Yorkshire crossbred gilts culled owing to vaginal discharge problem were collected from two commercial swine herds in Thailand. The estrous stage and gross pathology were examined. Blood samples were collected from the jugular vein prior to being slaughtered. Serum P
4
and cortisol were analyzed by means of enzyme immunoassay. The samples observed were in inactive (
n
= 4), follicular (
n
= 10), and luteal (
n
= 25) phases. They, afterwards, were processed in hematoxylin and eosin sections. The endometrium of the gilts was histologically divided into three layers, i.e., epithelial, subepithelial connective tissue, and glandular connective tissue layers. Immune cells, i.e., lymphocytes, neutrophils, eosinophils, macrophages, and plasma cells, in each layer were quantified under a light microscope (×400). The results revealed that mean serum cortisol was 430.6 ± 68.3 nmol/l. Serum P
4
varied by ovarian status. Serum P
4
of the gilts in the luteal phase was higher than those in the follicular phase (88.3 ± 7.7 versus 20.6 ± 6.2 nmol/l,
P <
0.05). As for the endometrium condition, the gilts were classified into acute/subacute endometritis (
n
= 13), chronic endometritis (
n
= 9), and normal endometrium (
n
= 17). Neutrophils were the main local immune cells in the epithelial layer. Lymphocytes were the dominant population in the subepithelial and glandular connective tissue layers. Generally, the serum cortisol tended to negatively correlate with lymphocytes in the subepithelial connective tissue layer (
r
= −0.28,
P =
0.081). In the gilts with acute/subacute endometritis, no correlation among serum cortisol, P
4
, and immune cells was observed. In chronic endometritis gilts, only a negative correlation was remarked between P
4
and epithelial lymphocytes (
r
= −0.83,
P =
0.010), epithelial neutrophils (
r
= −0.79,
P =
0.019), and subepithelial neutrophils (
r
= −0.73,
P =
0.025). In the gilts with normal endometrium, P
4
negatively correlated with subepithelial neutrophils (
r
= −0.55,
P =
0.022) while positively correlated with subepithelial macrophages (
r
= 0.54,
P =
0.024) and subepithelial eosinophils (
r
= 0.60,
P =
0.011).</abstract><cop>London</cop><pub>Springer-Verlag</pub><doi>10.1007/s00580-010-1033-2</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-5641 |
| ispartof | Comparative clinical pathology, 2011-12, Vol.20 (6), p.563-571 |
| issn | 1618-5641 1618-565X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1439235712 |
| source | Springer Nature |
| subjects | Hematology Medicine Medicine & Public Health Oncology Original Article Pathology |
| title | Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T07%3A55%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Association%20between%20serum%20cortisol%20and%20progesterone%20concentrations%20and%20the%20infiltration%20of%20immune%20cells%20in%20the%20endometrium%20of%20gilts%20with%20vaginal%20discharge&rft.jtitle=Comparative%20clinical%20pathology&rft.au=Roongsitthichai,%20Atthaporn&rft.date=2011-12&rft.volume=20&rft.issue=6&rft.spage=563&rft.epage=571&rft.pages=563-571&rft.issn=1618-5641&rft.eissn=1618-565X&rft_id=info:doi/10.1007/s00580-010-1033-2&rft_dat=%3Cproquest_cross%3E1439235712%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c2642-d8531aea3e1851c8a46b54b07f3c2747be39d7f5d4d824c38fac03a4ba0f03023%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1095628001&rft_id=info:pmid/&rfr_iscdi=true |