Expression of the cell-surface heparan sulfate proteoglycan syndecan-2 in developing rat anterior pituitary gland

• Published in: Cell and tissue research 2013-09, Vol.353 (3), p.473-481
• Main Authors: Horiguchi, Kotaro, Syaidah, Rahimi, Fujiwara, Ken, Tsukada, Takehiro, Ramadhani, Dini, Jindatip, Depicha, Kikuchi, Motoshi, Yashiro, Takashi
• Format: Article
• Language: English
• Subjects: adults; Animals; anterior pituitary; Biomedical and Life Sciences; Biomedicine; Cadherins - biosynthesis; Cadherins - genetics; Cellular biology; E-Cadherin; extracellular matrix; Gene expression; Gene Expression Regulation - physiology; genes; heparan sulfate; Human Genetics; Immunohistochemistry; Ki-67 Antigen - biosynthesis; Ki-67 Antigen - genetics; messenger RNA; Molecular Medicine; Pituitary gland; Pituitary Gland, Anterior - cytology; Pituitary Gland, Anterior - embryology; polymerase chain reaction; Proteins; proteoglycans; Proteomics; Rats; Rats, Wistar; Regular Article; Reverse Transcriptase Polymerase Chain Reaction; reverse transcription; Rodents; Sulfates; Syndecan-2 - biosynthesis; Syndecan-2 - genetics
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-013-1641-7

In the anterior pituitary gland, folliculo-stellate cells and five types of hormone-producing cells are surrounded by an extracellular matrix (ECM) essential for these cells to perform their respective roles. Syndecans—type I transmembrane cell-surface heparan sulfate proteoglycans act as major ECM coreceptors via their respective heparan sulfate chains and efficiently transduce intracellular signals through the convergent action of their transmembrane and cytoplasmic domains. The syndecans comprise four family members in vertebrates: syndecan-1, −2, −3 and −4. However, whether syndecans are produced in the pituitary gland or whether they have a role as a coreceptor is not known. We therefore used (1) reverse transcription plus the polymerase chain reaction to analyze the expression of syndecan genes and (2) immunohistochemical techniques to identify the cells that produce the syndecans in the anterior pituitary gland of adult rat. Syndecan-2 mRNA expression was clearly detected in the corticotropes of the anterior pituitary gland. Moreover, the expression of syndecan-2 in the developing pituitary gland had a distinct temporospatial pattern. To identify the cells expressing syndecan-2 in the developing pituitary gland, we used double-immunohistochemistry for syndecan-2 and the cell markers E-cadherin (immature cells) and Ki-67 (proliferating cells). Some E-cadherin– and Ki-67–immunopositive cells expressed syndecan-2. Therefore, syndecan-2 expression occurs in developmentally regulated patterns and syndecan-2 probably has different roles in adult and developing anterior pituitary glands.