Inulinase production by Geotrichum candidum OC-7 using migratory locusts as a new substrate and optimization process with Taguchi DOE

Utilization of migratory locusts (Locusta migratoria) as a main substrate due to its high protein content for inulinase (2,1-β-d-fructan fructanohydrolase) production by Geotrichum candidum OC-7 was investigated in this study. To optimize fermentation conditions, four influential factors (locust pow...

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Bibliographic Details
Published in:Toxicology and industrial health 2013-09, Vol.29 (8), p.704-710
Main Authors: Canli, Ozden, Tasar, Gani Erhan, Taskin, Mesut
Format: Article
Language:English
Subjects:
Animals
Bioreactors - microbiology
Biotechnology - methods
Culture Media - chemistry
Design of experiments
Enzymes
Fermentation
Geotrichum - enzymology
Geotrichum - growth & development
Geotrichum candidum
Glycoside Hydrolases - biosynthesis
Grasshoppers
Hydrogen-Ion Concentration
Insects
Locusta migratoria
Methods
Molecular biology
Optimization
Proteins
Research Design
Sucrose
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Summary:Utilization of migratory locusts (Locusta migratoria) as a main substrate due to its high protein content for inulinase (2,1-β-d-fructan fructanohydrolase) production by Geotrichum candidum OC-7 was investigated in this study. To optimize fermentation conditions, four influential factors (locust powder (LP) concentration, sucrose concentration, pH and fermentation time) at three levels were investigated using Taguchi orthogonal array (OA) design of experiment (DOE). Inulinase yield obtained from the designed experiments with regard to Taguchi L9 OA was processed with Minitab 15 software at ‘larger is better’ as quality character. The results showed that optimal fermentation conditions determined as LP 30 g/l, sucrose 20 g/l, pH 6.0 and time 48 h. Maximum inulinase activity was recorded as 30.12 U/ml, which was closer to the predicted value (30.56 U/ml). To verify the results, analysis of variance test was employed. LP had the greatest contribution (71.96%) among the other factors. Sucrose had lower contribution (13.96%) than LP. This result demonstrated that LP had a strong effect on inulinase activity and can be used for enzyme production. Taguchi DOE application enhanced enzyme activity to about 3.05-fold versus unoptimized condition and 2.34-fold versus control medium. Consequently, higher inulinase production can be achieved by the utilization of an edible insect material as an alternative substrate and Taguchi DOE presents suitable optimization method for biotechnological process.
ISSN:0748-2337
1477-0393
DOI:10.1177/0748233712442737