RNA interference by feeding in vitro–synthesized double‐stranded RNA to planarians: Methodology and dynamics

COVER PHOTOGRAPH: Low magnification image of planarian flatworms (Schmidtea mediterranea) fed with liver paste containing either red, green, yellow, or blue food dye. Uptake of colored liver paste containing in vitro synthesized double‐stranded RNA is readily visualized in the planarian gut. Ingesti...

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Bibliographic Details
Published in:Developmental dynamics 2013-06, Vol.242 (6), p.C1-C1
Main Authors: Rouhana, Labib, Weiss, Jennifer A., Forsthoefel, David J., Lee, Hayoung, King, Ryan S., Inoue, Takeshi, Shibata, Norito, Agata, Kiyokazu, Newmark, Phillip A.
Format: Article
Language:English
Subjects:
Dugesia japonica
Infections
planarian
Ribonucleic acid
RNA
RNAi
RNA‐interference
Schmidtea mediterranea
siRNA
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Summary:COVER PHOTOGRAPH: Low magnification image of planarian flatworms (Schmidtea mediterranea) fed with liver paste containing either red, green, yellow, or blue food dye. Uptake of colored liver paste containing in vitro synthesized double‐stranded RNA is readily visualized in the planarian gut. Ingestion and processing of double‐stranded RNA in the planarian gut leads to systemic RNA‐interference. Image credit: Labib Rouhana and Phillip A. Newmark, Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana‐Champaign. From RNA Interference by feeding in vitro‐synthesized double‐stranded RNA to planarians, Labib Rouhana, Jennifer A. Weiss, David J. Forsthoefel, Hayoung Lee, Ryan S. King, Takeshi Inoue, Norito Shibata, Kiyokazu Agata, and Phillip A. Newmark, Developmental Dynamics 242:718–730, 2013.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.23982