Characterization of monoclonal antibodies raised to Streptococcus mutans

Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans on...

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Bibliographic Details
Published in:Current microbiology 1985-01, Vol.12 (4), p.197-201
Main Authors: DOYLE, G, EVERHART, D
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
monoclonal antibodies
Streptococcus mutans
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Summary:Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans only (Mab-SM). Western blot analysis has shown that MAB-SM recognizes a 14,000-dalton component present in a partially purified membrane fraction recovered following glass-bead breakage of S. mutans P-4 cells. Two of the MAb-HT were found to react with a 35,000-dalton component from this same preparation.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01573331