Loading…

Characterization of monoclonal antibodies raised to Streptococcus mutans

Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans on...

Full description

Saved in:

Bibliographic Details
Published in:	Current microbiology 1985-01, Vol.12 (4), p.197-201
Main Authors:	DOYLE, G, EVERHART, D
Format:	Article
Language:	English
Subjects:	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Microbiology monoclonal antibodies Streptococcus mutans
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by
cites
container_end_page	201
container_issue	4
container_start_page	197
container_title	Current microbiology
container_volume	12
creator	DOYLE, G EVERHART, D
description	Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans only (Mab-SM). Western blot analysis has shown that MAB-SM recognizes a 14,000-dalton component present in a partially purified membrane fraction recovered following glass-bead breakage of S. mutans P-4 cells. Two of the MAb-HT were found to react with a 35,000-dalton component from this same preparation.
doi_str_mv	10.1007/BF01573331
format	article
fullrecord	<record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_14397401</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14397401</sourcerecordid><originalsourceid>FETCH-LOGICAL-p213t-ade74678d188a8b4cb2b495babdf8ccb1fed980a7154e87d898d91b24d9e69793</originalsourceid><addsrcrecordid>eNotj81KxDAYAIMouK5efIIcxFs1X5M2yVEX1xUWPKjn8uWnGGmbmqQHfXoX3NNchoEh5BrYHTAm7x-3DBrJOYcTsgLB64ppDadkxbjglWobOCcXOX8xBrVmsCK7zScmtMWn8IslxInGno5xinaIEw4UpxJMdMFnmjBk72iJ9K0kP5doo7VLpuNScMqX5KzHIfurI9fkY_v0vtlV-9fnl83Dvppr4KVC56VopXKgFCojrKmN0I1B43plrYHeO60YSmiEV9IprZwGUwunfaul5mty-9-dU_xefC7dGLL1w4CTj0vuDtNaCgYH8eYoYrY49AknG3I3pzBi-ulU2-qa1fwPdqtctQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14397401</pqid></control><display><type>article</type><title>Characterization of monoclonal antibodies raised to Streptococcus mutans</title><source>Springer LINK Archives</source><creator>DOYLE, G ; EVERHART, D</creator><creatorcontrib>DOYLE, G ; EVERHART, D</creatorcontrib><description>Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans only (Mab-SM). Western blot analysis has shown that MAB-SM recognizes a 14,000-dalton component present in a partially purified membrane fraction recovered following glass-bead breakage of S. mutans P-4 cells. Two of the MAb-HT were found to react with a 35,000-dalton component from this same preparation.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/BF01573331</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Microbiology ; monoclonal antibodies ; Streptococcus mutans</subject><ispartof>Current microbiology, 1985-01, Vol.12 (4), p.197-201</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8669202$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>DOYLE, G</creatorcontrib><creatorcontrib>EVERHART, D</creatorcontrib><title>Characterization of monoclonal antibodies raised to Streptococcus mutans</title><title>Current microbiology</title><description>Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans only (Mab-SM). Western blot analysis has shown that MAB-SM recognizes a 14,000-dalton component present in a partially purified membrane fraction recovered following glass-bead breakage of S. mutans P-4 cells. Two of the MAb-HT were found to react with a 35,000-dalton component from this same preparation.</description><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>monoclonal antibodies</subject><subject>Streptococcus mutans</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNotj81KxDAYAIMouK5efIIcxFs1X5M2yVEX1xUWPKjn8uWnGGmbmqQHfXoX3NNchoEh5BrYHTAm7x-3DBrJOYcTsgLB64ppDadkxbjglWobOCcXOX8xBrVmsCK7zScmtMWn8IslxInGno5xinaIEw4UpxJMdMFnmjBk72iJ9K0kP5doo7VLpuNScMqX5KzHIfurI9fkY_v0vtlV-9fnl83Dvppr4KVC56VopXKgFCojrKmN0I1B43plrYHeO60YSmiEV9IprZwGUwunfaul5mty-9-dU_xefC7dGLL1w4CTj0vuDtNaCgYH8eYoYrY49AknG3I3pzBi-ulU2-qa1fwPdqtctQ</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>DOYLE, G</creator><creator>EVERHART, D</creator><general>Springer</general><scope>IQODW</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>19850101</creationdate><title>Characterization of monoclonal antibodies raised to Streptococcus mutans</title><author>DOYLE, G ; EVERHART, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p213t-ade74678d188a8b4cb2b495babdf8ccb1fed980a7154e87d898d91b24d9e69793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>monoclonal antibodies</topic><topic>Streptococcus mutans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DOYLE, G</creatorcontrib><creatorcontrib>EVERHART, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DOYLE, G</au><au>EVERHART, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of monoclonal antibodies raised to Streptococcus mutans</atitle><jtitle>Current microbiology</jtitle><date>1985-01-01</date><risdate>1985</risdate><volume>12</volume><issue>4</issue><spage>197</spage><epage>201</epage><pages>197-201</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>Monoclonal antibodies (MAb) to whole cells of Streptococcus mutans P-4 (serotype e) were generated. Of the four MAb used in this investigation, three were found to cross-react with mouse heart tissue (MAb-HT) in the solid-phase ELISA, while the remaining MAb was found to be specific for S. mutans only (Mab-SM). Western blot analysis has shown that MAB-SM recognizes a 14,000-dalton component present in a partially purified membrane fraction recovered following glass-bead breakage of S. mutans P-4 cells. Two of the MAb-HT were found to react with a 35,000-dalton component from this same preparation.</abstract><cop>New York, NY</cop><pub>Springer</pub><doi>10.1007/BF01573331</doi><tpages>5</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0343-8651
ispartof	Current microbiology, 1985-01, Vol.12 (4), p.197-201
issn	0343-8651 1432-0991
language	eng
recordid	cdi_proquest_miscellaneous_14397401
source	Springer LINK Archives
subjects	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Microbiology monoclonal antibodies Streptococcus mutans
title	Characterization of monoclonal antibodies raised to Streptococcus mutans
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T03%3A54%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20monoclonal%20antibodies%20raised%20to%20Streptococcus%20mutans&rft.jtitle=Current%20microbiology&rft.au=DOYLE,%20G&rft.date=1985-01-01&rft.volume=12&rft.issue=4&rft.spage=197&rft.epage=201&rft.pages=197-201&rft.issn=0343-8651&rft.eissn=1432-0991&rft.coden=CUMIDD&rft_id=info:doi/10.1007/BF01573331&rft_dat=%3Cproquest_pasca%3E14397401%3C/proquest_pasca%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p213t-ade74678d188a8b4cb2b495babdf8ccb1fed980a7154e87d898d91b24d9e69793%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=14397401&rft_id=info:pmid/&rfr_iscdi=true