Cellular uptake and cytotoxicity of positively charged chitosan gold nanoparticles in human lung adenocarcinoma cells

Cellular uptake, cytotoxicity, and mechanisms of cytotoxicity of the positively charged Au nanoparticles (NPs) were examined in A549 cells, which are one of the most characterized pulmonary cellular systems. Positively charged Au NPs were prepared by chemical reduction using chitosan. The dimension...

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Published in:Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2012-12, Vol.14 (12), p.1-13, Article 1234
Main Authors: Choi, Seon Young, Jang, Soo Hwa, Park, Jin, Jeong, Saeromi, Park, Jin Ho, Ock, Kwang Su, Lee, Kangtaek, Yang, Sung Ik, Joo, Sang-Woo, Ryu, Pan Dong, Lee, So Yeong
Format: Article
Language:English
Subjects:
Cellular
Characterization and Evaluation of Materials
Charging
Chemical reduction
Chemistry and Materials Science
Chitosan
Cytotoxicity
Dynamics
Gold
Inorganic Chemistry
Lasers
Light scattering
Materials Science
Nanoparticles
Nanotechnology
Optical Devices
Optics
Photonics
Physical Chemistry
Research Paper
Transmission electron microscopy
Uptakes
Zeta potential
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Summary:Cellular uptake, cytotoxicity, and mechanisms of cytotoxicity of the positively charged Au nanoparticles (NPs) were examined in A549 cells, which are one of the most characterized pulmonary cellular systems. Positively charged Au NPs were prepared by chemical reduction using chitosan. The dimension and surface charge of Au NPs were examined by transmission electron microscopy (TEM), dynamic light scattering, and zeta potential measurements. The uptake of Au NPs into A549 cells was also monitored using TEM and dark-field microscopy (DFM) and z-stack confocal microRaman spectroscopy. DFM live cell imaging was also performed to monitor the entry of chitosan Au NPs in real time. The cytotoxic assay, using both methylthiazol tetrazolium and lactate dehydrogenase assays revealed that positively charged Au NPs decreased cell viability. Flow cytometry, DNA fragmentation, real-time PCR, and western blot analysis suggest that positively charged chitosan Au NPs provoke cell damage through both apoptotic and necrotic pathways.
ISSN:1388-0764
1572-896X
DOI:10.1007/s11051-012-1234-5