Microfluidic devices for the isolation of circulating rare cells: A focus on affinity-based, dielectrophoresis, and hydrophoresis

Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells is used for cancer diagnosis and prognosis, while DNA analysis or enumeration of nucleated red blood cells is useful for prenatal diagn...

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Bibliographic Details
Published in:Electrophoresis 2013-04, Vol.34 (7), p.1028-1041
Main Authors: Hyun, Kyung-A, Jung, Hyo-Il
Format: Article
Language:English
Subjects:
Affinity-based isolation
Animals
Cancer
Cell Separation - instrumentation
Cell Separation - methods
Circulating
Circulating rare cells
Devices
Diagnosis
Dielectrophoresis
Diseases
Electric Conductivity
Electrophoresis
Electrophoresis - instrumentation
Electrophoresis - methods
Enumeration
Humans
Label-free separation
MCF-7 Cells
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidic device
Microfluidics
Neoplastic Cells, Circulating - pathology
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Summary:Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells is used for cancer diagnosis and prognosis, while DNA analysis or enumeration of nucleated red blood cells is useful for prenatal diagnosis or hypoxic anemia, and that of circulating stem cells to diagnose cancer metastasis. Isolation of these cells and their downstream analyses can provide significant information such as the origin and characteristics of a disease. Novel approaches based on microfluidics have many advantages, including the continuous process and integration with other components for analysis. For these reasons, a variety of microfluidic devices have been developed to isolate and characterize rare cells. In this article, we review several microfluidic devices, with a focus on affinity‐based isolation (e.g. antigen‐antibody reaction) and label‐free separation (DEP and hydrophoresis).
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201200417