Real Time In Vivo Measurement of Ascorbate in the Brain Using Carbon Nanotube-Modified Microelectrodes

A carbon fiber microelectrode modified with a composite film of carbon nanotubes and Nafion was developed for in vivo ascorbate (AA) measurements in brain tissue. The modified‐microelectrodes exhibit an electrocatalytic activity for AA oxidation by shifting the peak potential negatively to −0.040 V,...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2013-07, Vol.25 (7), p.1757-1763
Main Authors: Ferreira, Nuno R., Santos, Ricardo M., Laranjinha, João, Barbosa, Rui M.
Format: Article
Language:English
Subjects:
Ascorbate
Biocompatibility
Biomedical materials
Brain
Carbon fiber microelectrodes
Carbon nanotubes
In vivo electrochemistry
In vivo testing
In vivo tests
Microelectrodes
Nanostructure
Surgical implants
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Summary:A carbon fiber microelectrode modified with a composite film of carbon nanotubes and Nafion was developed for in vivo ascorbate (AA) measurements in brain tissue. The modified‐microelectrodes exhibit an electrocatalytic activity for AA oxidation by shifting the peak potential negatively to −0.040 V, showing a sensitivity of 37 pA/µM, a detection limit of 2.5 µM, a response time of 0.3 s and don’t respond to several electroactive compounds found in the brain extracellular space. In the rat hippocampus, the basal concentration of AA was 290 µM, and glutamate‐evoked changes in AA were biphasic comprising fast and slow components.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201300053