Rapid analysis of amino acids using pre-column derivatization

A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino a...

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Bibliographic Details
Published in:Journal of Chromatography A 1984-01, Vol.336 (1), p.93-104
Main Authors: Bidlingmeyer, Brian A., Cohen, Steven A., Tarvin, Thomas L.
Format: Article
Language:English
Subjects:
amino acids
Applied sciences
Exact sciences and technology
liquid chromatography
Other techniques and industries
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Summary:A new approach to the pre-column derivatization and analysis of amino acids is described. The method is based upon formation of a phenylthiocarbamyl derivative of the amino acids. The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydorlyzates. The liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids with 12-min analysis time and a 1-pmol sensitivity.
ISSN:0378-4347
0021-9673
DOI:10.1016/S0378-4347(00)85133-6