Application of reversed-phase high-performance liquid chromatography using pre-column derivatization with o-phthaldialdehyde for the quantitative analysis of amino acids in adult and fetal sheep plasma, animal feeds and tissues

The concentration of amino acids in adult and fetal sheep plasma, liver, muscle and animal feeds was determined by reversed-phase high-performance liquid chromatography using an automated data acquisition system and pre-column o-phthaldialdehyde derivatization. Of the seventeen amino acids in the st...

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Bibliographic Details
Published in:Journal of Chromatography A 1984, Vol.315, p.321-331
Main Authors: Krishnamurti, C.R., Heindze, A.M., Galzy, G.
Format: Article
Language:English
Subjects:
amino acids
Applied sciences
Exact sciences and technology
high-performance liquid chromatography
o-phthaldialdehyde
Other techniques and industries
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Summary:The concentration of amino acids in adult and fetal sheep plasma, liver, muscle and animal feeds was determined by reversed-phase high-performance liquid chromatography using an automated data acquisition system and pre-column o-phthaldialdehyde derivatization. Of the seventeen amino acids in the standard mixture, fifteen including glycine and threonine were completely resolved in a total analysis time of 50 min. The clear resolution, high degree of precision and accuracy, relatively rapid analysis and the lack of interference from chemical contaminants in feed and tissue hydrolysates render this technique suitable for routine analysis of large numbers of biological samples of nutritional interest.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)90749-6