New application of high-performance liquid chromatography for analysis of urinary C-peptide

A successful application of high-performance liquid chromatography for analysis of urinary C-peptide is described. Samples (1.0 ml of human urine) were first subjected to gel chromatography to remove interfering substances, and then applied to a reversed-phase column (LiChrosorb RP-18, 7 μm). The de...

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Bibliographic Details
Published in:Journal of Chromatography A 1985-01, Vol.338 (1), p.71-78
Main Authors: Oyama, Hideki, Endoh, Masahiko, Yoneda, Masaya, Matsuki, Michihiro, Satoh, Atsuko, Nishida, Seikoh, Horino, Masaharu
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
high-performance liquid chromatography
man
peptides
Proteins
urine
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Summary:A successful application of high-performance liquid chromatography for analysis of urinary C-peptide is described. Samples (1.0 ml of human urine) were first subjected to gel chromatography to remove interfering substances, and then applied to a reversed-phase column (LiChrosorb RP-18, 7 μm). The detection of C-peptide was performed using a highly specific radioimmunoassay. With the newly developed techniques, at least four forms of immunoreactive C-peptide were detected in human urine. One of these peptides was indistinguishable from authentic C-peptide. The present study has clearly demonstrated the heterogeneity of urinary C-peptide.
ISSN:0378-4347
0021-9673
DOI:10.1016/0378-4347(85)80071-2