Screening for a "new" enzyme in nature: Haloperoxidase production by Death Valley dematiaceous Hyphomycetes

Haloperoxidases are enzymes that have the ability to halogenate a broad range of substrates. To find a biologically produced haloperoxidase that could function at a pH greater than 3.0 and at a temperature greater than 19 degree C, dematiaceous hyphomycetes were isolated from the Death Valley desert...

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Bibliographic Details
Main Authors: Hunter-Cevera, J C, Sotos, L
Format: Book Chapter
Language:English
Subjects:
Drechslera halodes
haloperoxidase
heat tolerance
reviews
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Summary:Haloperoxidases are enzymes that have the ability to halogenate a broad range of substrates. To find a biologically produced haloperoxidase that could function at a pH greater than 3.0 and at a temperature greater than 19 degree C, dematiaceous hyphomycetes were isolated from the Death Valley desert and screened for their ability to produce such an enzyme. A qualitative assay using bromophenol red was employed in situ over a 12-day fermentation period. Several dematiaceous hyphomycetes, such as Dreschlera haloides and Ulocladium chartarum , produced haloperoxidases that were active in broth culture at 19, 15, and 34 degree C at pH 7.0 and 8.0.
ISSN:0095-3628